Department of Dermatology, Kyoto University Graduate School of Medicine, Kyoto, Japan.
Methods Mol Biol. 2021;2223:151-157. doi: 10.1007/978-1-0716-1001-5_11.
The regulation of vascular permeability is critical in inflammation. It controls the distribution of water and plasma contents such as immunoglobulins in peripheral tissues. To regulate allergic diseases, it is important to study vascular biology especially in inflammation. Since the vascular permeability changes in minutes upon the exposure to proinflammatory mediators, intravital imaging system is a powerful technique to capture such dynamic responses. We here describe how to evaluate vascular permeability in vivo using multiphoton microscopy. We use various sizes of fluorescence-labeled dextran to visualize how leaky the blood vessels are in the steady state and in inflammation. Using this assay system, we can illustrate the dynamic kinetics of vascular permeability in vivo in real-time. This assay system provides a novel convenient way to study vascular biology that is beneficial in the assessment of various animal models of allergic disease.
血管通透性的调节在炎症中至关重要。它控制着水和免疫球蛋白等血浆成分在周围组织中的分布。为了调节过敏疾病，研究血管生物学，特别是炎症中的血管生物学非常重要。由于血管通透性在接触促炎介质后的几分钟内就会发生变化，因此活体成像系统是捕捉这种动态反应的有力技术。我们在这里描述如何使用多光子显微镜评估体内血管通透性。我们使用各种大小的荧光标记葡聚糖来观察血管在稳定状态和炎症中的通透性。使用这种检测系统，我们可以实时直观显示体内血管通透性的动态动力学。该检测系统为研究血管生物学提供了一种新颖便捷的方法，有助于评估各种过敏性疾病的动物模型。
