对小鼠肺部支气管肺泡灌洗液的细胞和生化分析。
Cellular and Biochemical Analysis of Bronchoalveolar Lavage Fluid from Murine Lungs.
机构信息
Department of Pharmaceutical Sciences, College of Health Professions, School of Pharmacy, North Dakota State University, Fargo, ND, USA.
出版信息
Methods Mol Biol. 2021;2223:201-215. doi: 10.1007/978-1-0716-1001-5_15.
Abstract
Bronchoalveolar lavage (BAL) is a technique used to collect the contents of the airways. The fluid recovered, called BAL fluid (BALF), serves as a dynamic tool to identify various disease pathologies ranging from asthma to infectious diseases to cancer in the lungs. A wide array of tests can be performed with BALF, including total and differential leukocyte counts (DLC), enzyme-linked immunosorbent assays (ELISA) or flow-cytometric quantitation of inflammatory mediators, such as cytokines, chemokines and adhesion molecules, and assessment of nitrate and nitrite content for estimation of nitric oxide synthase (NOS) activity. Here, we describe a detailed procedure for the collection of BALF for a variety of downstream usages, including DLC by cytological and flow-cytometry-based methods, multiplex cytokine analysis by flow cytometry, and NOS activity analysis by determining nitrate and nitrite levels.
摘要
支气管肺泡灌洗（BAL）是一种用于收集气道内容物的技术。回收的液体称为 BAL 液（BALF），可作为一种动态工具，用于识别从哮喘到肺部传染病到癌症等各种疾病的病理。可以通过 BALF 进行多种测试，包括总白细胞计数和分类计数（DLC）、酶联免疫吸附测定（ELISA）或流式细胞术定量炎性介质，如细胞因子、趋化因子和粘附分子，以及评估硝酸盐和亚硝酸盐含量以估计一氧化氮合酶（NOS）活性。在这里，我们描述了一种详细的 BALF 收集程序，用于各种下游用途，包括通过细胞学和基于流式细胞术的方法进行 DLC、通过流式细胞术进行多重细胞因子分析以及通过确定硝酸盐和亚硝酸盐水平来分析 NOS 活性。
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