McIntire Erik, Leonhard Kimberly, Taapken Seth, Larson Anna Lisa
University of Chicago, Chicago, IL, USA.
WiCell Research Institute, Madison, WI, USA.
Methods Mol Biol. 2021;2239:251-268. doi: 10.1007/978-1-0716-1084-8_16.
Acquired chromosomal abnormalities may occur during the reprogramming and culture of human pluripotent stem cells (hPSCs). Therefore, it is required that regular testing of genetic integrity be conducted. G-banded karyotyping is a widely used genetic assay that is capable of detecting chromosomal abnormalities. Karyotyping of hPSC cultures can be a challenging undertaking for inexperienced investigators; here, we provide detailed procedures for karyotyping, including sample preparation and analysis, as well as the interpretation of hPSC karyotype results.
在人类多能干细胞（hPSC）的重编程和培养过程中可能会出现获得性染色体异常。因此，需要定期进行基因完整性检测。G带核型分析是一种广泛使用的基因检测方法，能够检测染色体异常。对于缺乏经验的研究人员来说，hPSC培养物的核型分析可能是一项具有挑战性的工作；在此，我们提供了核型分析的详细程序，包括样本制备和分析，以及hPSC核型结果的解读。
