Collaborative Innovation Center of Seafood Deep Processing, Zhejiang Province Joint Key Laboratory of Aquatic Products Processing, Institute of Seafood, Zhejiang Gongshang University, Hangzhou, 310013, China.
College of Standardization, China Jiliang University, Hangzhou 310018, China.
J Agric Food Chem. 2020 Dec 9;68(49):14652-14659. doi: 10.1021/acs.jafc.0c06675. Epub 2020 Nov 23.
Eicosapentaenoic and docosahexaenoic acids structured phospholipids (PL) have multiple biochemical and pharmacological effects on human health. In this study, EPA and DHA chains were locked under precursor ion scan (PreIS) mode for untargeted screening PL in krill oil using hydrophilic interaction liquid chromatography tandem mass spectrometry (HILIC-MS/MS). The effect of collision energy and declustering potential on the fragmentation of EPA ( 301.2) and DHA ( 327.2) chains was studied. A total of 33 PL were characterized (sn-1/sn-2) and quantified using regression models, including 16 PC, 11 PE, and 6 PI. Afterward, this method was validated in terms of linearity (≥0.9978), sensitivity (LOD ≤ 4.02 μg·L), precision (RSD ≤ 4.71%), and recovery (≥78.9%). Finally, the performance of HILIC-PreIS-MS/MS was compared with those of conventional methods, and the results indicated its superiority in selective screening PL in krill oil.
二十碳五烯酸和二十二碳六烯酸结构磷脂 (PL) 对人类健康具有多种生化和药理学作用。在这项研究中，使用亲水作用色谱串联质谱法 (HILIC-MS/MS)，通过前体离子扫描 (PreIS) 模式将 EPA 和 DHA 链锁定在磷虾油中的非靶向 PL 筛选中。研究了碰撞能和去簇电位对 EPA（301.2）和 DHA（327.2）链的碎片化的影响。使用回归模型对 33 种 PL（sn-1/sn-2）进行了特征描述和定量，其中包括 16 种 PC、11 种 PE 和 6 种 PI。然后，从线性（≥0.9978）、灵敏度（LOD≤4.02μg·L）、精密度（RSD≤4.71%）和回收率（≥78.9%）方面对该方法进行了验证。最后，将 HILIC-PreIS-MS/MS 的性能与常规方法进行了比较，结果表明其在磷虾油中选择性筛选 PL 方面具有优越性。
