Oya-Silva Laís Fernanda, Vicari Taynah, Rodrigo Disner Geonildo, Lirola Juliana Roratto, Klingelfus Tatiane, Gonçalves Helyandra de Lourdes Schicora, Leite Thalita Pires Borges, Calado Sabrina Loise de Morais, Voigt Carmen Lúcia, Silva de Assis Helena Cristina, Cestari Marta Margarete
Department of Genetics, Laboratory of Animal Cytogenetics and Environmental Mutagenesis, Federal University of Paraná (UFPR), Curitiba, Paraná, Brazil.
Department of Pharmacology, Laboratory of Aquatic Toxicology, Federal University of Paraná (UFPR), Curitiba, Paraná, Brazil.
Environ Toxicol Pharmacol. 2021 Feb;82:103551. doi: 10.1016/j.etap.2020.103551. Epub 2020 Nov 20.
The aquatic environment is the major recipient of wastes containing nanoparticles and other contaminants. Titanium dioxide nanoparticles (NPTiO) are one of the most produced and used nanoparticle worldwide. This study investigated the toxicity of NPTiO, as well as the toxicity interaction between NPTiO and lead (Pb), in response to genetic and biochemical biomarkers using freshwater fish Rhamdia quelen, as an animal model. The results showed genotoxicity in blood and kidney tissues. No effect of NPTiO alone or in co-exposure with Pb on liver genotoxicity were observed. Alterations in the antioxidant hepatic enzymes activities, as well as alterations in glutathione levels indicated that NPTiO alone or in co-exposure with Pb can cause antioxidant imbalance. The lipid peroxidation was also raised after exposure to NPTiO. In general, the results of this study indicated that both NPTiO alone and their co-exposure with Pb are capable of producing significant toxic effects in short-term exposure.
水生环境是含有纳米颗粒和其他污染物的废物的主要接纳者。二氧化钛纳米颗粒（NPTiO）是全球产量和使用量最大的纳米颗粒之一。本研究以淡水鱼奎氏溪蟾鱼作为动物模型，利用遗传和生化生物标志物，研究了NPTiO的毒性以及NPTiO与铅（Pb）之间的毒性相互作用。结果显示血液和肾脏组织存在遗传毒性。未观察到单独的NPTiO或与Pb共同暴露对肝脏遗传毒性有影响。肝脏抗氧化酶活性的改变以及谷胱甘肽水平的改变表明，单独的NPTiO或与Pb共同暴露会导致抗氧化失衡。暴露于NPTiO后脂质过氧化也有所增加。总体而言，本研究结果表明，单独的NPTiO及其与Pb的共同暴露在短期暴露中均能产生显著的毒性作用。
