Sharma Ritu, Jindal Rajinder, Jhamb Nikhil, Banaee Mahdi, Faggio Caterina
Department of Biosciences, University Institute of Biotechnology, Chandigarh University, Gharuan, Punjab, India.
Aquatic Biology Laboratory, Department of Zoology, Panjab University, Chandigarh, India.
Microsc Res Tech. 2025 Mar;88(3):810-817. doi: 10.1002/jemt.24748. Epub 2024 Nov 23.
The toxic impact of environmentally relevant nominal sub-lethal concentration of lead nitrate (23 mg/L) on the gills of Cyprinus carpio after 30 days of exposure was assessed. Structural alterations were analyzed through histopathology, and the DNA damage rate in peripheral erythrocytes was evaluated by alkaline comet assay. A significant deviation in the gill histoarchitecture was observed compared to the control group. Significant changes, including the curling of secondary lamellae, loss of filaments, necrosis, hyperplasia, hypertrophy of cells of primary filament, and vacuolization, were found in the fish exposed to nominal sub-lethal concentration of lead nitrate. Moreover, the comet assay showed lead nitrate-induced DNA damage, evidenced by the length of the DNA "tail" in the exposed fish. The findings of this study strongly indicated that lead nitrate, even at sub-lethal levels, could significantly alter the overall physiology of the fish. This situation could lead to severe ecological consequences.
评估了环境相关的标称亚致死浓度硝酸铅（23毫克/升）暴露30天后对鲤鱼鳃的毒性影响。通过组织病理学分析结构改变，并通过碱性彗星试验评估外周血红细胞中的DNA损伤率。与对照组相比，观察到鳃组织结构有显著偏差。在暴露于标称亚致死浓度硝酸铅的鱼中发现了显著变化，包括次生鳃小片卷曲、鳃丝缺失、坏死、增生、初级鳃丝细胞肥大和空泡化。此外，彗星试验显示硝酸铅诱导了DNA损伤，暴露鱼中DNA“尾巴”的长度证明了这一点。本研究结果强烈表明，即使在亚致死水平，硝酸铅也会显著改变鱼的整体生理状况。这种情况可能导致严重的生态后果。
