Ribeiro Sofia, Carvalho Ana M, Fernandes Emanuel M, Gomes Manuela E, Reis Rui L, Bayon Yves, Zeugolis Dimitrios I
Sofradim Production, Medtronic, Trevoux, France; Regenerative, Modular & Developmental Engineering Laboratory (REMODEL), National University of Ireland Galway (NUI Galway), Galway, Ireland; Science Foundation Ireland (SFI) Centre for Research in Medical Devices (CÚRAM) National University of Ireland Galway (NUI Galway), Galway, Ireland.
3B's Research Group, I3Bs - Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Barco, Guimarães, Portugal; ICVS/3B's - PT Government Associate Laboratory, Braga / Guimarães, Portugal.
Acta Biomater. 2021 Feb;121:303-315. doi: 10.1016/j.actbio.2020.11.026. Epub 2020 Nov 20.
Although it has been repeatedly indicated the importance to develop implantable devices and cell culture substrates with tissue-specific rigidity, current commercially available products, in particular cell culture substrates, have rigidity values well above most tissues in the body. Herein, six resorbable polyester films were fabricated using compression moulding with a thermal presser into films with tailored stiffness by appropriately selecting the ratio of their building up monomers (e.g. lactide, glycolide, trimethylene carbonate, dioxanone, ε-caprolactone). Typical NMR and FTIR spectra were obtained, suggesting that the fabrication process did not have a negative effect on the conformation of the polymers. Surface roughness analysis revealed no apparent differences between the films as a function of polymer composition. Subject to polymer composition, polymeric films were obtained with glass transition temperatures from -52 °C to 61 °C; contact angles in water from 81 ° to 94 °; storage modulus from 108 MPa to 2,756 MPa and loss modulus from 8 MPa to 507 MPa (both in wet state, at 1 Hz frequency and at 37 °C); ultimate tensile strength from 8 MPa to 62 MPa, toughness from 23 MJ/m to 287 MJ/m, strain at break from 3 % to 278 %, macro-scale Young's modulus from 110 MPa to 2,184 MPa (all in wet state); and nano-scale Young's modulus from 6 kPa to 15,019 kPa (in wet state). With respect to in vitro degradation in phosphate buffered saline at 37 °C, some polymeric films [e.g. poly(glycolide-lactide) 30 / 70] started degrading from day 7 (shortest timepoint assessed), whilst others [e.g. poly(glycolide-co-ε-caprolactone) 10 / 90] were more resilient to degradation up to day 21 (longest timepoint assessed). In vitro biological analysis using human dermal fibroblasts and a human monocyte cell line (THP-1) showed the potential of the polymeric films to support cell growth and controlled immune response. Evidently, the selected polymers exhibited properties suitable for a range of clinical indications.
尽管已多次指出开发具有组织特异性刚度的可植入设备和细胞培养底物的重要性，但目前市售产品，尤其是细胞培养底物，其刚度值远高于体内大多数组织。在此，通过热压机采用压缩成型法制备了六种可吸收聚酯薄膜，通过适当选择其组成单体（如丙交酯、乙交酯、三亚甲基碳酸酯、二氧杂环己酮、ε-己内酯）的比例来制备具有定制刚度的薄膜。获得了典型的核磁共振和傅里叶变换红外光谱，表明制备过程对聚合物的构象没有负面影响。表面粗糙度分析显示，薄膜之间没有因聚合物组成而产生明显差异。根据聚合物组成，获得的聚合物薄膜的玻璃化转变温度为-52℃至61℃；在水中的接触角为81°至94°；储能模量为108MPa至2756MPa，损耗模量为8MPa至507MPa（均在湿态、1Hz频率和37℃下）；极限拉伸强度为8MPa至62MPa，韧性为23MJ/m至287MJ/m，断裂应变从3%至278%，宏观尺度杨氏模量为110MPa至2184MPa（均在湿态）；纳米尺度杨氏模量为6kPa至15019kPa（在湿态）。关于在37℃的磷酸盐缓冲盐水中的体外降解，一些聚合物薄膜[如聚（乙交酯-丙交酯）30/70]从第7天（评估的最短时间点）开始降解，而其他薄膜[如聚（乙交酯-共-ε-己内酯）10/90]在长达第21天（评估的最长时间点）对降解更具弹性。使用人皮肤成纤维细胞和人单核细胞系（THP-1）进行的体外生物学分析表明，聚合物薄膜具有支持细胞生长和控制免疫反应的潜力。显然，所选聚合物表现出适合一系列临床适应症的特性。
