Leibniz Institute for Analytical Sciences (ISAS), 44139 Dortmund, Germany.
Sensors (Basel). 2020 Nov 19;20(22):6611. doi: 10.3390/s20226611.
Nanoparticle Tracking Analysis (NTA) allows for the simultaneous determination of both size and concentration of nanoparticles in a sample. This study investigates the accuracy of particle size and concentration measurements performed on an LM10 device. For experiments, standard nanoparticles of different sizes composed of two materials with different refractive indices were used. Particle size measurements were found to have a decent degree of accuracy. This fact was verified by the manufacturer-reported particle size-determined by transmission electron microscopy (TEM)-as well as by performed scanning electron microscopy (SEM) measurements. On the other hand, concentration measurements resulted in overestimation of the particle concentration in majority of cases. Thus, our findings confirmed the accuracy of nanoparticle sizing performed by the LM10 instrument and highlighted the overestimation of particle concentration made by this device. In addition, an approach of swift correction of the results of concentration measurements received for samples is suggested in the presented study.
纳米颗粒跟踪分析（NTA）可同时测定样品中纳米颗粒的大小和浓度。本研究考察了在 LM10 仪器上进行的颗粒大小和浓度测量的准确性。实验中，使用了由两种具有不同折射率的材料组成的不同尺寸的标准纳米颗粒。研究发现，颗粒大小测量具有相当高的准确性。这一事实得到了制造商报告的通过透射电子显微镜（TEM）确定的颗粒大小以及通过进行的扫描电子显微镜（SEM）测量的验证。另一方面，浓度测量结果在大多数情况下导致颗粒浓度的高估。因此，我们的研究结果证实了 LM10 仪器进行的纳米颗粒尺寸测量的准确性，并强调了该设备对颗粒浓度的高估。此外，本研究还提出了一种快速校正样品浓度测量结果的方法。
