Bhatia A L, Tausch H, Stehlik G
Institute of Biology, Austrian Research Centre Seibersdorf.
Ecotoxicol Environ Saf. 1987 Aug;14(1):48-55. doi: 10.1016/0147-6513(87)90082-0.
The catalytic chlorination of polycyclic aromatic hydrocarbons leads to the formation of complex mixtures of isomers analogous to those found in fly ash samples of an incineration plant for radioactive waste. Therefore chlorination mixtures of chrysene, pyrene, and fluoranthene were analyzed and characterized by gas chromatography-mass spectrometry. Determination of the exact position of each chlorosubstituent within the molecule was not possible either by mass spectrometry or by comparison with well-defined methyl analogs. The toxic as well as mutagenic effects of such mixtures of isomers was compared with those of their parent hydrocarbons in the microtiter fluctuation test using Salmonella typhimurium TA 98 and TA 100 in a concentration range of 0.5 to 5.0 microgram/ml. No toxic effect could be observed, but the chlorinated products in contrast to their parent compounds were found to be strong mutagens to the S. typhimurium test strains and showed a positive response even without enzymatic activation. Frameshift mutations as well as base pair alterations were detected.
多环芳烃的催化氯化会导致形成异构体的复杂混合物，类似于在放射性废物焚烧厂的飞灰样品中发现的那些。因此，对芘、荧蒽和屈的氯化混合物进行了气相色谱 - 质谱分析和表征。无论是通过质谱法还是与明确的甲基类似物进行比较，都无法确定分子内每个氯取代基的确切位置。在微滴波动试验中，使用鼠伤寒沙门氏菌TA 98和TA 100，在0.5至5.0微克/毫升的浓度范围内，将这种异构体混合物的毒性和诱变作用与其母体烃的毒性和诱变作用进行了比较。未观察到毒性作用，但与它们的母体化合物相比，氯化产物被发现对鼠伤寒沙门氏菌测试菌株具有很强的诱变作用，并且即使没有酶促激活也表现出阳性反应。检测到了移码突变以及碱基对改变。
