Australian Institute for Bioengineering and Nanotechnology, the University of Queensland, Brisbane, QLD 4072, Australia.
Queensland Micro-and Nanotechnology Centre, Griffith University, Brisbane, QLD 4111, Australia.
Biosensors (Basel). 2020 Nov 19;10(11):182. doi: 10.3390/bios10110182.
Microfluidic lab-on-a-chip cell culture techniques have been gaining popularity by offering the possibility of reducing the amount of samples and reagents and greater control over cellular microenvironment. Polydimethylsiloxane (PDMS) is the commonly used polymer for microfluidic cell culture devices because of the cheap and easy fabrication techniques, non-toxicity, biocompatibility, high gas permeability, and optical transparency. However, the intrinsic hydrophobic nature of PDMS makes cell seeding challenging when applied on PDMS surface. The hydrophobicity of the PDMS surface also allows the non-specific absorption/adsorption of small molecules and biomolecules that might affect the cellular behaviour and functions. Hydrophilic modification of PDMS surface is indispensable for successful cell seeding. This review collates different techniques with their advantages and disadvantages that have been used to improve PDMS hydrophilicity to facilitate endothelial cells seeding in PDMS devices.
微流控芯片实验室细胞培养技术因其能够减少样品和试剂的用量以及更好地控制细胞微环境而受到越来越多的关注。聚二甲基硅氧烷（PDMS）因其廉价且易于制造、无毒、生物相容性好、透气性高和光学透明等特点，成为微流控细胞培养器件中常用的聚合物。然而，PDMS 的固有疏水性使得在 PDMS 表面进行细胞接种具有挑战性。PDMS 表面的疏水性还允许小分子和生物分子的非特异性吸附/吸收，这可能会影响细胞的行为和功能。PDMS 表面的亲水性修饰对于成功接种细胞是必不可少的。本文综述了不同的技术及其优缺点，这些技术已被用于提高 PDMS 的亲水性，以促进内皮细胞在 PDMS 器件中的接种。
