The State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
College of Life Science and Technology, Mudanjiang Normal University, Mudanjiang, 157012, China.
BMC Biol. 2024 May 20;22(1):116. doi: 10.1186/s12915-024-01913-1.
Verticillium wilt, caused by the fungus Verticillium dahliae, is a soil-borne vascular fungal disease, which has caused great losses to cotton yield and quality worldwide. The strain KRS010 was isolated from the seed of Verticillium wilt-resistant Gossypium hirsutum cultivar "Zhongzhimian No. 2."
The strain KRS010 has a broad-spectrum antifungal activity to various pathogenic fungi as Verticillium dahliae, Botrytis cinerea, Fusarium spp., Colletotrichum spp., and Magnaporthe oryzae, of which the inhibition rate of V. dahliae mycelial growth was 73.97% and 84.39% respectively through confrontation test and volatile organic compounds (VOCs) treatments. The strain was identified as Bacillus altitudinis by phylogenetic analysis based on complete genome sequences, and the strain physio-biochemical characteristics were detected, including growth-promoting ability and active enzymes. Moreover, the control efficiency of KRS010 against Verticillium wilt of cotton was 93.59%. After treatment with KRS010 culture, the biomass of V. dahliae was reduced. The biomass of V. dahliae in the control group (Vd991 alone) was 30.76-folds higher than that in the treatment group (KRS010+Vd991). From a molecular biological aspect, KRS010 could trigger plant immunity by inducing systemic resistance (ISR) activated by salicylic acid (SA) and jasmonic acid (JA) signaling pathways. Its extracellular metabolites and VOCs inhibited the melanin biosynthesis of V. dahliae. In addition, KRS010 had been characterized as the ability to promote plant growth.
This study indicated that B. altitudinis KRS010 is a beneficial microbe with a potential for controlling Verticillium wilt of cotton, as well as promoting plant growth.
由真菌黄萎轮枝孢（Verticillium dahliae）引起的黄萎病是一种土传维管束真菌病害，已在全球范围内对棉花的产量和品质造成了巨大损失。菌株 KRS010 是从抗黄萎病棉花品种“中棉 2 号”种子中分离得到的。
菌株 KRS010 对多种病原菌具有广谱抗真菌活性，包括黄萎轮枝孢、灰葡萄孢、镰刀菌属、炭疽菌属和稻瘟病菌，对峙试验和挥发性有机化合物（VOCs）处理分别导致黄萎轮枝孢菌丝生长抑制率达到 73.97%和 84.39%。基于全基因组序列的系统发育分析将该菌株鉴定为高山芽孢杆菌，检测了该菌株的生理生化特性，包括促生能力和活性酶。此外，KRS010 对棉花黄萎病的防治效率为 93.59%。用 KRS010 培养液处理后，黄萎轮枝孢的生物量减少。对照组（Vd991 单独）的黄萎轮枝孢生物量是处理组（KRS010+Vd991）的 30.76 倍。从分子生物学角度来看，KRS010 通过诱导水杨酸（SA）和茉莉酸（JA）信号通路激活的系统抗性（ISR）触发植物免疫。其胞外代谢物和 VOCs 抑制了黄萎轮枝孢黑色素的生物合成。此外，KRS010 已被证明具有促进植物生长的能力。
本研究表明，高山芽孢杆菌 KRS010 是一种有益的微生物，具有防治棉花黄萎病和促进植物生长的潜力。
