Department of Nursing, Chang Gung University of Science and Technology, Chia-Yi County, Taiwan.
Department of Anesthesiology, Chang Gung Memorial Hospital, Chiayi County, Taiwan.
BMC Complement Med Ther. 2020 Nov 23;20(1):364. doi: 10.1186/s12906-020-03158-1.
Arctium lappa L. root (burdock root) has long been recommended for the treatment of different diseases in traditional Chinese medicine. Burdock root possesses anti-oxidative, anti-inflammatory, anti-cancer, and anti-microbial activities. The aim of the study was to elucidate whether aqueous extract of burdock root regulates mesenchymal stem cell proliferation and differentiation.
Human bone marrow-derived mesenchymal stem cells in 2D high density culture and in 3D micromass pellets were treated with chondrogenic induction medium and chondral basal medium in the absence or presence of aqueous extract of burdock root. The chondrogenic differentiation was accessed by staining glucosaminoglycans, immunostaining SOX9 and type II collagen and immuonblotting of SOX9, aggrecan and type II collagen.
Treatment of aqueous extract of burdock root increased the cell proliferation of hMSCs. It did not have significant effect on osteogenic and adipogenic differentiation, but significantly enhanced chondrogenic induction medium-induced chondrogenesis. The increment was dose dependent, as examined by staining glucosaminoglycans, SOX9, and type II collagen and immunobloting of SOX9, aggrecan and type II collagen in 2D and 3D cultures. In the presence of supplemental materials, burdock root aqueous extract showed equivalent chondrogenic induction capability to that of TGF-β.
The results demonstrate that aqueous extract of Arctium lappa L. root promotes chondrogenic medium-induced chondrogenic differentiation. The aqueous extract of burdock root can even be used alone to stimulate chondrogenic differentiation. The study suggests that the aqueous extract of burdock root can be used as an alternative strategy for treatment purposes.
牛蒡根（牛蒡根）长期以来一直被推荐用于治疗传统中药中的各种疾病。牛蒡根具有抗氧化、抗炎、抗癌和抗菌活性。本研究旨在阐明牛蒡根的水提取物是否调节间充质干细胞的增殖和分化。
在不存在或存在牛蒡根水提取物的情况下，用软骨形成诱导培养基和软骨基础培养基处理二维高密度培养和三维微团培养的人骨髓来源间充质干细胞。通过染色糖胺聚糖、免疫染色 SOX9 和 II 型胶原以及 SOX9、聚集蛋白聚糖和 II 型胶原的免疫印迹来评估软骨分化。
牛蒡根水提取物处理可增加 hMSCs 的细胞增殖。它对成骨和脂肪分化没有显著影响，但显著增强了软骨形成诱导培养基诱导的软骨形成。通过在二维和三维培养物中染色糖胺聚糖、SOX9 和 II 型胶原以及免疫印迹 SOX9、聚集蛋白聚糖和 II 型胶原来检查，增加与剂量有关。在补充材料的存在下，牛蒡根水提取物显示出与 TGF-β 相当的软骨形成诱导能力。
结果表明，牛蒡根的水提取物促进软骨形成培养基诱导的软骨分化。牛蒡根的水提取物甚至可以单独用于刺激软骨分化。该研究表明，牛蒡根的水提取物可作为治疗目的的替代策略。
