• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

植物乳杆菌 YYC-3 的代谢产物可能通过抑制 VEGF-MMP2/9 信号通路抑制结肠癌转移。

Metabolite secretions of Lactobacillus plantarum YYC-3 may inhibit colon cancer cell metastasis by suppressing the VEGF-MMP2/9 signaling pathway.

机构信息

Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, People's Republic of China.

College of Food Science, Northeast Agricultural University, Harbin, 150030, People's Republic of China.

出版信息

Microb Cell Fact. 2020 Nov 23;19(1):213. doi: 10.1186/s12934-020-01466-2.

DOI:10.1186/s12934-020-01466-2
PMID:33228670
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7684877/
Abstract

BACKGROUND

Colorectal cancer (CRC) is a major clinical challenge, and the gut microbiome plays important roles in the occurrence and metastasis of CRC. Lactobacillus and their metabolites are thought to be able to suppress the growth of CRC cells. However, the antimetastatic mechanism of Lactobacillus or their metabolites toward CRC cells is not clear. Therefore, the aim of this study was to assess the inhibitory mechanism of cell-free supernatants (CFSs) of L. rhamnosus GG, L. casei M3, and L. plantarum YYC-3 on metastasis of CRC cells.

RESULTS

YYC-3 CFS showed the highest inhibitory effect on CRC cell growth, invasion and migration, and inhibited MMP2, MMP9, and VEGFA gene and protein expression, and protein secretion. Furthermore, it suppressed the activities of MMPs by gelatin zymography. Moreover, the effective compounds in these CFSs were analyzed by Q Exactive Focus liquid chromatography-mass spectrometry.

CONCLUSIONS

Our results showed that metabolite secretions of YYC-3 may inhibited cell metastasis by downregulating the VEGF/MMPs signaling pathway. These data suggest that treatment of CRC cells with metabolites from L. plantarum YYC-3 may reduce colon cancer metastasis.

摘要

背景

结直肠癌（CRC）是一个主要的临床挑战，肠道微生物群在 CRC 的发生和转移中起着重要作用。人们认为乳酸菌及其代谢产物能够抑制 CRC 细胞的生长。然而，乳酸菌或其代谢产物对 CRC 细胞的抗转移机制尚不清楚。因此，本研究旨在评估鼠李糖乳杆菌 GG、干酪乳杆菌 M3 和植物乳杆菌 YYC-3 的无细胞上清液（CFS）对 CRC 细胞转移的抑制机制。

结果

YYC-3 CFS 对 CRC 细胞生长、侵袭和迁移具有最高的抑制作用，并抑制 MMP2、MMP9 和 VEGFA 基因和蛋白表达以及蛋白分泌。此外，它通过明胶酶谱法抑制 MMPs 的活性。此外，通过 Q Exactive Focus 液相色谱-质谱法分析了这些 CFS 中的有效化合物。

结论

我们的结果表明，YYC-3 的代谢产物分泌可能通过下调 VEGF/MMPs 信号通路抑制细胞转移。这些数据表明，用植物乳杆菌 YYC-3 的代谢产物处理 CRC 细胞可能会降低结肠癌的转移。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1753/7684877/2ae8f96391f7/12934_2020_1466_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1753/7684877/3f610303fd02/12934_2020_1466_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1753/7684877/83d9644cbf93/12934_2020_1466_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1753/7684877/58fd7fc7aa70/12934_2020_1466_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1753/7684877/4adcc9026fb8/12934_2020_1466_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1753/7684877/b28aac19d448/12934_2020_1466_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1753/7684877/2ae8f96391f7/12934_2020_1466_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1753/7684877/3f610303fd02/12934_2020_1466_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1753/7684877/83d9644cbf93/12934_2020_1466_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1753/7684877/58fd7fc7aa70/12934_2020_1466_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1753/7684877/4adcc9026fb8/12934_2020_1466_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1753/7684877/b28aac19d448/12934_2020_1466_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1753/7684877/2ae8f96391f7/12934_2020_1466_Fig6_HTML.jpg

相似文献

1
Metabolite secretions of Lactobacillus plantarum YYC-3 may inhibit colon cancer cell metastasis by suppressing the VEGF-MMP2/9 signaling pathway.植物乳杆菌 YYC-3 的代谢产物可能通过抑制 VEGF-MMP2/9 信号通路抑制结肠癌转移。
Microb Cell Fact. 2020 Nov 23;19(1):213. doi: 10.1186/s12934-020-01466-2.
2
Probiotic strain Lactobacillus plantarum YYC-3 prevents colon cancer in mice by regulating the tumour microenvironment.植物乳杆菌 YYC-3 通过调节肿瘤微环境预防小鼠结肠癌。
Biomed Pharmacother. 2020 Jul;127:110159. doi: 10.1016/j.biopha.2020.110159. Epub 2020 Apr 27.
3
Cell-free supernatants from probiotic Lactobacillus casei and Lactobacillus rhamnosus GG decrease colon cancer cell invasion in vitro.益生菌干酪乳杆菌和鼠李糖乳杆菌 GG 的无细胞上清液可降低体外结肠癌细胞的侵袭能力。
Nutr Cancer. 2012 Aug;64(6):871-8. doi: 10.1080/01635581.2012.700758. Epub 2012 Jul 25.
4
Tumor-targeted induction of intrinsic apoptosis in colon cancer cells by Lactobacillus plantarum and Lactobacillus rhamnosus strains.植物乳杆菌和鼠李糖乳杆菌菌株通过诱导内在凋亡靶向结肠癌肿瘤。
Mol Biol Rep. 2023 Jun;50(6):5345-5354. doi: 10.1007/s11033-023-08445-x. Epub 2023 May 8.
5
Matrix metalloproteinase 2 promotes cell growth and invasion in colorectal cancer.基质金属蛋白酶 2 促进结直肠癌中的细胞生长和侵袭。
Acta Biochim Biophys Sin (Shanghai). 2011 Nov;43(11):840-8. doi: 10.1093/abbs/gmr085. Epub 2011 Oct 3.
6
Integrated Multi-Omics Investigations of Metalloproteinases in Colon Cancer: Focus on MMP2 and MMP9.结肠癌中金属蛋白酶的综合多组学研究：聚焦基质金属蛋白酶2和基质金属蛋白酶9
Int J Mol Sci. 2021 Nov 17;22(22):12389. doi: 10.3390/ijms222212389.
7
GABA-producing Lactobacillus plantarum inhibits metastatic properties and induces apoptosis of 5-FU-resistant colorectal cancer cells via GABA receptor signaling.产 GABA 的植物乳杆菌通过 GABA 受体信号抑制 5-FU 耐药结直肠癌细胞的转移特性并诱导其凋亡。
J Microbiol. 2021 Feb;59(2):202-216. doi: 10.1007/s12275-021-0562-5. Epub 2021 Feb 1.
8
The effect of five probiotic lactobacilli strains on the growth and biofilm formation of Streptococcus mutans.五种益生菌乳酸杆菌菌株对变形链球菌生长和生物膜形成的影响。
Oral Dis. 2015 Jan;21(1):e128-34. doi: 10.1111/odi.12257. Epub 2014 Jun 3.
9
Volatile profile of elderberry juice: Effect of lactic acid fermentation using L. plantarum, L. rhamnosus and L. casei strains.接骨木果原汁的挥发性成分分析：利用植物乳杆菌、鼠李糖乳杆菌和干酪乳杆菌进行乳酸发酵的影响。
Food Res Int. 2018 Mar;105:412-422. doi: 10.1016/j.foodres.2017.11.042. Epub 2017 Nov 22.
10
Effects of 4 Probiotic Strains in Coculture with Traditional Starters on the Flavor Profile of Yogurt.4种益生菌菌株与传统发酵剂共培养对酸奶风味特征的影响。
J Food Sci. 2017 Jul;82(7):1693-1701. doi: 10.1111/1750-3841.13779. Epub 2017 Jun 9.

引用本文的文献

1
Unveiling a sustainable approach to cancer treatment: the antitumor activity of amygdalin and cell-free supernatant of Lacticaseibacillus rhamnosus.揭示一种可持续的癌症治疗方法：苦杏仁苷和鼠李糖乳杆菌无细胞上清液的抗肿瘤活性
Folia Microbiol (Praha). 2025 Jul 10. doi: 10.1007/s12223-025-01289-x.
2
Probiotics and Cancer: Mechanistic Insights and Organ-Specific Impact.益生菌与癌症：机制洞察及器官特异性影响
Biomolecules. 2025 Jun 16;15(6):879. doi: 10.3390/biom15060879.
3
Microbiome in cancer metastasis: biological insights and emerging spatial omics methods.

本文引用的文献

1
Probiotic strain Lactobacillus plantarum YYC-3 prevents colon cancer in mice by regulating the tumour microenvironment.植物乳杆菌 YYC-3 通过调节肿瘤微环境预防小鼠结肠癌。
Biomed Pharmacother. 2020 Jul;127:110159. doi: 10.1016/j.biopha.2020.110159. Epub 2020 Apr 27.
2
Stable Colonization of Orally Administered SY13 Alters the Gut Microbiota.口服给予 SY13 可稳定定植并改变肠道微生物群。
Biomed Res Int. 2020 Feb 13;2020:5281639. doi: 10.1155/2020/5281639. eCollection 2020.
3
Inhibitory effects of Lactobacilli cocktail on HT-29 colon carcinoma cells growth and modulation of the Notch and Wnt/β-catenin signaling pathways.
癌症转移中的微生物组：生物学见解与新兴的空间组学方法。
Front Cell Infect Microbiol. 2025 Jun 4;15:1559870. doi: 10.3389/fcimb.2025.1559870. eCollection 2025.
4
The interaction network between intestinal flora and cell death in microecosystem of pan-cancers.全癌微生态系统中肠道菌群与细胞死亡之间的相互作用网络。
Int J Med Sci. 2025 May 16;22(11):2596-2608. doi: 10.7150/ijms.111723. eCollection 2025.
5
Matrix Dynamics and Microbiome Crosstalk: Matrix Metalloproteinases as Key Players in Disease and Therapy.基质动力学与微生物组相互作用：基质金属蛋白酶在疾病与治疗中的关键作用
Int J Mol Sci. 2025 Apr 11;26(8):3621. doi: 10.3390/ijms26083621.
6
The human microbiome: redefining cancer pathogenesis and therapy.人类微生物组：重新定义癌症发病机制与治疗
Cancer Cell Int. 2025 Apr 28;25(1):165. doi: 10.1186/s12935-025-03787-x.
7
exerts anticancer effects and increase the chemosensitivity of 5-fluorouracil against oral cancer cells .发挥抗癌作用并增强5-氟尿嘧啶对口腔癌细胞的化学敏感性。
Bioimpacts. 2024 Aug 3;15:30427. doi: 10.34172/bi.30427. eCollection 2025.
8
Melanoma Cell Adhesion Molecule Plays a Pivotal Role in Proliferation, Migration, Tumor Immune Microenvironment, and Immunotherapy in Colorectal Cancer.黑色素瘤细胞粘附分子在结直肠癌的增殖、迁移、肿瘤免疫微环境及免疫治疗中起关键作用。
Cancer Med. 2025 Mar;14(5):e70740. doi: 10.1002/cam4.70740.
9
Relationship Between MIC-1, VEGF, and TGF-β1 and Clinicopathologic Stage and Lymph Node Metastasis in Gastric Cancer.胃癌中巨噬细胞抑制因子-1、血管内皮生长因子和转化生长因子-β1与临床病理分期及淋巴结转移的关系
Int J Gen Med. 2025 Feb 21;18:955-965. doi: 10.2147/IJGM.S497572. eCollection 2025.
10
The interplay between gut bacteria and targeted therapies: implications for future cancer treatments.肠道细菌与靶向治疗之间的相互作用：对未来癌症治疗的启示。
Mol Med. 2025 Feb 13;31(1):58. doi: 10.1186/s10020-025-01108-6.
嗜酸乳杆菌混合物对 HT-29 结肠癌细胞生长的抑制作用及对 Notch 和 Wnt/β-连环蛋白信号通路的调节作用。
Microb Pathog. 2020 Feb;139:103829. doi: 10.1016/j.micpath.2019.103829. Epub 2019 Nov 2.
4
Characterization of the Anti-Cancer Activity of the Probiotic Bacterium Using 2D vs. 3D Culture in Colorectal Cancer Cells.利用 2D 与 3D 培养比较益生菌细菌在结直肠癌细胞中的抗癌活性。
Biomolecules. 2019 Oct 1;9(10):557. doi: 10.3390/biom9100557.
5
Anti-Colorectal Cancer Effects of Probiotic-Derived p8 Protein.益生菌衍生的 p8 蛋白对结直肠癌的防治作用。
Genes (Basel). 2019 Aug 19;10(8):624. doi: 10.3390/genes10080624.
6
Zinc oxide nanoparticle induced neurotoxic potential upon interaction with primary astrocytes.氧化锌纳米颗粒与原代星形胶质细胞相互作用引起的神经毒性潜力。
Neurotoxicology. 2019 Jul;73:213-227. doi: 10.1016/j.neuro.2019.04.008. Epub 2019 Apr 25.
7
Sperm matrix metalloproteinase-2 activity increased in pregnant couples treated with intrauterine insemination: a prospective case control study.宫腔内人工授精治疗的妊娠夫妇精子基质金属蛋白酶-2活性增加：一项前瞻性病例对照研究。
J Obstet Gynaecol. 2019 Jul;39(5):675-680. doi: 10.1080/01443615.2018.1558189. Epub 2019 Mar 28.
8
Global, regional, and national burden of brain and other CNS cancer, 1990-2016: a systematic analysis for the Global Burden of Disease Study 2016.全球、区域和国家脑和其他中枢神经系统癌症负担，1990-2016 年：2016 年全球疾病负担研究的系统分析。
Lancet Neurol. 2019 Apr;18(4):376-393. doi: 10.1016/S1474-4422(18)30468-X. Epub 2019 Feb 21.
9
LncRNA XIST facilitates proliferation and epithelial-mesenchymal transition of colorectal cancer cells through targeting miR-486-5p and promoting neuropilin-2.LncRNA XIST 通过靶向 miR-486-5p 并促进神经纤毛蛋白-2 促进结直肠癌细胞的增殖和上皮-间充质转化。
J Cell Physiol. 2019 Aug;234(8):13747-13761. doi: 10.1002/jcp.28054. Epub 2019 Jan 17.
10
K5 displays adhesion, anti-proliferative activity and apoptotic effects in human colon cancer cells.K5 在人结肠癌细胞中显示出黏附、抗增殖活性和凋亡作用。
Benef Microbes. 2018 Dec 7;9(6):975-983. doi: 10.3920/BM2017.0183. Epub 2018 Oct 24.