Bank H L
Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston.
Diabetologia. 1987 Oct;30(10):812-6. doi: 10.1007/BF00275748.
A rapid fluorometric method has been developed to evaluate the viability of isolated islet cells. The assay differentiates between viable and nonviable cells by the simultaneous use of the inclusion and exclusion dyes acridine orange and propidium iodide. When viewed by fluorescent microscopy, viable cells fluoresce green, while nonviable cells fluoresce bright red. Although the acridine orange and propidium iodide assay measures membrane integrity, the results of this assay correlate with other measures of cell viability. Compared to trypan blue exclusion, this assay is easier to read, more stable, and has fewer staining artifacts. The assay enables the rapid estimation of the viability of a population of islet cells prior to time-consuming experiments rather than retrospectively. This assay can also be used with intact islets. Stained islets can be divided into three distinct groups: green fluorescing islets contain insulin, red fluorescing islets contain little or no insulin and a third class of islets containing some non-viable cells fluoresce red, green, and yellow. The yellow colour is due to the superimposition of red and green fluorescing cells.
已开发出一种快速荧光法来评估分离的胰岛细胞的活力。该检测方法通过同时使用包含染料吖啶橙和排除染料碘化丙啶来区分活细胞和死细胞。通过荧光显微镜观察时，活细胞发出绿色荧光，而死细胞发出亮红色荧光。尽管吖啶橙和碘化丙啶检测法测量的是膜完整性，但该检测结果与其他细胞活力指标相关。与台盼蓝排除法相比，该检测方法更易于读取、更稳定且染色假象更少。该检测方法能够在进行耗时实验之前而非事后快速估计一群胰岛细胞的活力。此检测方法也可用于完整的胰岛。染色后的胰岛可分为三个不同的组：发出绿色荧光的胰岛含有胰岛素，发出红色荧光的胰岛几乎不含或不含胰岛素，第三类含有一些死细胞的胰岛发出红色、绿色和黄色荧光。黄色是由于发出红色和绿色荧光的细胞叠加所致。
