Pharmacognosy and Medicinal Plants Department, Faculty of Pharmacy (Girls), Al-Azhar University, Cairo, 11651, Egypt.
Department of Biochemistry, National Organization for Drug Control & Research, Cairo, Egypt.
Appl Biochem Biotechnol. 2023 Oct;195(10):5881-5902. doi: 10.1007/s12010-022-04292-x. Epub 2023 Jan 28.
Thunbergia erecta L. contains cytotoxic and liver-protective compounds. Thunbergia erecta L. leaves were macerated in 70% aqueous ethanol, then fractionated with ethyl acetate (9.3 g) and butanol (12.7 g), and attenuated Den-induced liver cancer in a Wistar rat experimental model. Ethyl acetate and butanol fractions were chromatographed using column chromatography and solid-phase extraction (SPE); Vicenin-II (1), kaempferol (2), biochanin A, sissotrin 7-O-β-glucopyranoside (3), gentianose (4), acacetin 7-O-β-glucopyranoside (5), apigenin 7-O-β-glucopyranoside (6), and rosmarinic acid (7) were extracted, and their structures were determined using NMR spectroscopy and ESI-mass spectrometry. Sixty rats were divided into six groups (ten each): control group, Den group, doxorubicin/Den-treated group, butanol fraction/Den-treated group, and isolated acacetin 7-O-β-glucopyranoside/Den-treated group. The liver enzymes and proinflammatory biomarkers were used to estimate the liver function. In addition, liver tissues were collected for analysis of oxidative stress markers, gene expression, and histopathology. There is a significant increase in the levels of liver enzymes, AFP, and TNF-ἁ. This was conveyed by a significant increase of IL-1 and caspase-3, elevation of MDA and reduction of GSH, and suppression of Bcl2 and elevation of Bax expression. All parameters in butanol, ethyl acetate fractions, and isolated acacetin 7-O-β-glucopyranoside (major constituents) of T. erecta L. were significantly improved to values close to those of the control group.
直立山牵牛含有细胞毒性和保肝化合物。将直立山牵牛的叶子在 70%的含水乙醇中浸渍，然后用乙酸乙酯（9.3 克）和正丁醇（12.7 克）进行分馏，并在 Wistar 大鼠实验模型中减弱 Den 诱导的肝癌。使用柱色谱法和固相萃取（SPE）对乙酸乙酯和正丁醇馏分进行色谱分离；从其中提取得到 vicenin-II（1）、山奈酚（2）、大豆素、sissotrin 7-O-β-吡喃葡萄糖苷（3）、龙胆二糖（4）、acacetin 7-O-β-吡喃葡萄糖苷（5）、芹菜素 7-O-β-吡喃葡萄糖苷（6）和迷迭香酸（7），并通过 NMR 光谱和 ESI 质谱确定其结构。将 60 只大鼠分为六组（每组 10 只）：对照组、Den 组、多柔比星/Den 处理组、正丁醇馏分/Den 处理组和分离的 acacetin 7-O-β-吡喃葡萄糖苷/Den 处理组。使用肝酶和促炎生物标志物来估计肝功能。此外，收集肝脏组织用于分析氧化应激标志物、基因表达和组织病理学。肝酶、AFP 和 TNF-α 的水平显著升高。这是通过 IL-1 和 caspase-3 的显著增加、MDA 的升高和 GSH 的降低以及 Bcl2 的抑制和 Bax 表达的升高来传达的。T. erecta L. 的正丁醇、乙酸乙酯馏分和分离的 acacetin 7-O-β-吡喃葡萄糖苷（主要成分）中的所有参数均显著改善，接近对照组的值。
