Shen Yang, Wang Wen Jun, Fu Ming, Xu Guo Qiang, Zhou Xiang, Liu Bang
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Yi Chuan. 2020 Oct 20;42(10):1028-1035. doi: 10.16288/j.yczz.20-108.
To identify the original components of Asini Colla Corii and its raw material hides provides a guarantee for authenticity of Asini Colla Corii. It is urgent for Asini Colla Corii production enterprises and market supervision departments to develop effective identification methods of Asini Colla Corii and hides derived from horses, donkeys, mules and hinnies. This study screened species-specific DNA sequences of nuclear and mitochondrial genomes as detection targets, designed horse and donkey specific primers and established multiple PCR identification methods for identifying the animal hides (including the horse, donkey, mule and hinny) and Asini Colla Corii containing horse-derived and donkey-derived components. Our method can identify the horse, donkey, mule and hinny hides and horse, donkey-derived components of Asini Colla Corii with high species specificity (no crossed amplification was observed ). The limit of detection was 0.2 ng DNA. The method developed in this study provides technical support for Asini Colla Corii production enterprises and market supervision departments.
鉴定阿胶及其原料皮的原始成分，为阿胶的真伪提供了保障。阿胶生产企业和市场监管部门迫切需要开发有效的阿胶及其马、驴、骡、马骡皮的鉴别方法。本研究筛选了核基因组和线粒体基因组的物种特异性DNA序列作为检测靶点，设计了马和驴特异性引物，建立了多重PCR鉴定方法，用于鉴定动物皮（包括马、驴、骡、马骡）以及含有马源和驴源成分的阿胶。我们的方法能够以高物种特异性鉴定出马、驴、骡、马骡皮以及阿胶中的马、驴源成分（未观察到交叉扩增）。检测限为0.2 ng DNA。本研究开发的方法为阿胶生产企业和市场监管部门提供了技术支持。
