Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, People's Republic of China.
Inspection and Testing Center of Agricultural and Livestock Products of Tibet, Lhasa 850000, People's Republic of China.
J Food Prot. 2021 Feb 1;84(2):194-199. doi: 10.4315/JFP-20-140.
Donkey hide gelatin (Colla corii asini) is well-known for its high nutritional value, especially for medicinal purposes. However, it is also a potential candidate for adulteration because of its low yield and high price. To quantitatively detect adulterated donkey hide gelatin with all possible mixed animal species, a real-time PCR approach on the basis of single-copy housekeeping nuclear reference primers was proposed in this study. For the system establishment, mixtures containing designated contents of pig hide with donkey hide were used to generate a calibration curve on the basis of the ratio of cycle threshold, CT (specificity/reference) with reasonable linearity (5 to 100%). Then, a set of experiments were performed on commercially available samples. The proposed PCR approach could specifically identify donkey hide from mixed animal products and quantify the content of donkey hide gelatin, thus facilitating control over this novel form of donkey hide gelatin adulteration.
驴皮明胶（阿胶）以其高营养价值而闻名，尤其在药用方面。然而，由于其产量低、价格高，它也是潜在的掺假目标。为了定量检测可能与所有混合动物物种掺假的驴皮明胶，本研究提出了一种基于单拷贝管家核参考引物的实时 PCR 方法。在系统建立过程中，使用含有指定含量猪皮与驴皮的混合物，根据循环阈值（特异性/参考）的比值生成一条具有合理线性（5 至 100%）的校准曲线。然后，在市售样品上进行了一系列实验。该 PCR 方法可以从混合动物产品中特异性地识别驴皮，并定量驴皮明胶的含量，从而有助于控制这种新型的驴皮明胶掺假。
