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周围神经损伤后近端和远端神经残端中细胞因子表达的生物信息学分析。

Bioinformatic analysis of cytokine expression in the proximal and distal nerve stumps after peripheral nerve injury.

作者信息

Cheng Xiao-Qing, Xu Wen-Jing, Ding Xiao, Han Gong-Hai, Wei Shuai, Liu Ping, Meng Hao-Ye, Shang Ai-Jia, Wang Yu, Wang Ai-Yuan

机构信息

Institute of Orthopedics; Beijing Key Laboratory of Regenerative Medicine in Orthopedics; Key Lab of Musculoskeletal Trauma & War Injuries, Chinese PLA General Hospital, Beijing, China.

Department of Neurosurgery, Chinese PLA General Hospital, Beijing; Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu Province, China.

出版信息

Neural Regen Res. 2021 May;16(5):878-884. doi: 10.4103/1673-5374.295348.

DOI:10.4103/1673-5374.295348
PMID:33229723
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8178785/
Abstract

In our previous study, we investigated the dynamic expression of cytokines in the distal nerve stumps after peripheral nerve injury using microarray analysis, which can characterize the dynamic expression of proteins. In the present study, we used a rat model of right sciatic nerve transection to examine changes in the expression of cytokines at 1, 7, 14 and 28 days after injury using protein microarray analysis. Interleukins were increased in the distal nerve stumps at 1-14 days post nerve transection. However, growth factors and growth factor-related proteins were mainly upregulated in the proximal nerve stumps. The P-values of the inflammatory response, apoptotic response and cell-cell adhesion in the distal stumps were higher than those in the proximal nerve stumps, but the opposite was observed for angiogenesis. The number of cytokines related to axons in the distal stumps was greater than that in the proximal stumps, while the percentage of cytokines related to axons in the distal stumps was lower than that in the proximal nerve stumps. Visualization of the results revealed the specific expression patterns and differences in cytokines in and between the proximal and distal nerve stumps. Our findings offer potential therapeutic targets and should help advance the development of clinical treatments for peripheral nerve injury. Approval for animal use in this study was obtained from the Animal Ethics Committee of the Chinese PLA General Hospital on September 7, 2016 (approval No. 2016-x9-07).

摘要

在我们之前的研究中，我们使用可对蛋白质动态表达进行表征的微阵列分析，研究了周围神经损伤后远端神经残端中细胞因子的动态表达。在本研究中，我们采用大鼠右侧坐骨神经横断模型，运用蛋白质微阵列分析检测损伤后1天、7天、14天和28天细胞因子表达的变化。神经横断后1 - 14天，远端神经残端中的白细胞介素增加。然而，生长因子和生长因子相关蛋白主要在近端神经残端中上调。远端残端中炎症反应、凋亡反应和细胞间黏附的P值高于近端神经残端，但血管生成情况则相反。远端残端中与轴突相关的细胞因子数量多于近端残端，而远端残端中与轴突相关的细胞因子百分比低于近端神经残端。结果可视化显示了近端和远端神经残端内及之间细胞因子的特定表达模式和差异。我们的研究结果提供了潜在的治疗靶点，应有助于推动周围神经损伤临床治疗的发展。本研究中动物使用的批准于2016年9月7日获得中国人民解放军总医院动物伦理委员会批准（批准号：2016 - x9 - 07）。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/848a/8178785/f3ca99868458/NRR-16-878-g007.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/848a/8178785/dc13205e213f/NRR-16-878-g003.jpg
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2
Different protein expression patterns in rat spinal nerves during Wallerian degeneration assessed using isobaric tags for relative and absolute quantitation proteomics profiling.使用同量异位标签相对和绝对定量蛋白质组学分析评估大鼠沃勒变性过程中脊髓神经的不同蛋白质表达模式。
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Metascape provides a biologist-oriented resource for the analysis of systems-level datasets.
Metascape 为系统水平数据集的分析提供了面向生物学家的资源。
Nat Commun. 2019 Apr 3;10(1):1523. doi: 10.1038/s41467-019-09234-6.
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CXCL9, CXCL10, CXCL11, and their receptor (CXCR3) in neuroinflammation and neurodegeneration.CXCL9、CXCL10、CXCL11及其受体（CXCR3）在神经炎症和神经退行性变中的作用
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Overlapping Mechanisms of Peripheral Nerve Regeneration and Angiogenesis Following Sciatic Nerve Transection.坐骨神经横断后周围神经再生与血管生成的重叠机制
Front Cell Neurosci. 2017 Oct 11;11:323. doi: 10.3389/fncel.2017.00323. eCollection 2017.
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Wallerian demyelination: chronicle of a cellular cataclysm.华勒氏变性：一场细胞灾难的编年史。
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