Wei Shuai, Liang Xue-Zhen, Hu Qian, Wang Wei-Shan, Xu Wen-Jing, Cheng Xiao-Qing, Peng Jiang, Guo Quan-Yi, Liu Shu-Yun, Jiang Wen, Ding Xiao, Han Gong-Hai, Liu Ping, Shi Chen-Hui, Wang Yu
The First Affiliated Hospital of Medical College, Shihezi University, Shihezi, Xinjiang Uygur Autonomous Region; Institute of Orthopedics, Chinese PLA General Hospital, Beijing; Co-Innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu Province, China.
Institute of Orthopedics, Chinese PLA General Hospital, Beijing; The First Clinical Medical School, Shandong University of Traditional Chinese Medicine, Jinan, Shandong Province, China.
Neural Regen Res. 2020 Feb;15(2):315-323. doi: 10.4103/1673-5374.265556.
Sensory and motor nerve fibers of peripheral nerves have different anatomies and regeneration functions after injury. To gain a clear understanding of the biological processes behind these differences, we used a labeling technique termed isobaric tags for relative and absolute quantitation to investigate the protein profiles of spinal nerve tissues from Sprague-Dawley rats. In response to Wallerian degeneration, a total of 626 proteins were screened in sensory nerves, of which 368 were upregulated and 258 were downregulated. In addition, 637 proteins were screened in motor nerves, of which 372 were upregulated and 265 were downregulated. All identified proteins were analyzed using the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis of bioinformatics, and the presence of several key proteins closely related to Wallerian degeneration were tested and verified using quantitative real-time polymerase chain reaction analyses. The differentially expressed proteins only identified in the sensory nerves were mainly relevant to various biological processes that included cell-cell adhesion, carbohydrate metabolic processes and cell adhesion, whereas differentially expressed proteins only identified in the motor nerves were mainly relevant to biological processes associated with the glycolytic process, cell redox homeostasis, and protein folding. In the aspect of the cellular component, the differentially expressed proteins in the sensory and motor nerves were commonly related to extracellular exosomes, the myelin sheath, and focal adhesion. According to the Kyoto Encyclopedia of Genes and Genomes, the differentially expressed proteins identified are primarily related to various types of metabolic pathways. In conclusion, the present study screened differentially expressed proteins to reveal more about the di?erences and similarities between sensory and motor nerves during Wallerian degeneration. The present findings could provide a reference point for a future investigation into the differences between sensory and motor nerves in Wallerian degeneration and the characteristics of peripheral nerve regeneration. The study was approved by the Ethics Committee of the Chinese PLA General Hospital, China (approval No. 2016-x9-07) in September 2016.
外周神经的感觉神经纤维和运动神经纤维在损伤后具有不同的解剖结构和再生功能。为了清楚地了解这些差异背后的生物学过程，我们使用了一种称为相对和绝对定量等压标签的标记技术，来研究来自Sprague-Dawley大鼠的脊神经组织的蛋白质谱。针对沃勒变性，在感觉神经中总共筛选出626种蛋白质，其中368种上调，258种下调。此外，在运动神经中筛选出637种蛋白质，其中372种上调，265种下调。所有鉴定出的蛋白质都使用基因本体论和京都基因与基因组百科全书进行生物信息学分析，并使用定量实时聚合酶链反应分析对几种与沃勒变性密切相关的关键蛋白质的存在进行了测试和验证。仅在感觉神经中鉴定出的差异表达蛋白质主要与各种生物学过程相关，包括细胞间粘附、碳水化合物代谢过程和细胞粘附，而仅在运动神经中鉴定出的差异表达蛋白质主要与糖酵解过程、细胞氧化还原稳态和蛋白质折叠相关的生物学过程有关。在细胞成分方面，感觉神经和运动神经中的差异表达蛋白质通常与细胞外囊泡、髓鞘和粘着斑有关。根据京都基因与基因组百科全书，鉴定出的差异表达蛋白质主要与各种类型的代谢途径有关。总之，本研究筛选了差异表达蛋白质，以揭示更多关于沃勒变性期间感觉神经和运动神经之间的差异和相似性。本研究结果可为未来研究沃勒变性中感觉神经和运动神经的差异以及周围神经再生的特征提供参考依据。该研究于2016年9月获得中国人民解放军总医院伦理委员会批准（批准号：2016-x9-07）。
