Salonen I, Kallajoki M
Department of Anatomy, University of Turku, Finland.
Int J Androl. 1987 Dec;10(6):731-9. doi: 10.1111/j.1365-2605.1987.tb00376.x.
Human spermatozoa were exposed to a monoclonal antibody (C11H), which recognizes sperm acrosin. The antibody was presented to the sperm during capacitation and/or insemination, and its effect on penetration was tested using zona-free hamster eggs. An inhibitory effect on penetration was observed when the antibody was present during insemination but not when it was included only in the capacitation medium. As judged by immunofluorescence microscopy, most of the sperm bound to the egg surface were devoid of acrosomal staining. Some of the bound sperm were stained at their equatorial segments. Sperm that had penetrated the ooplasm did not exhibit immunofluorescence.
将人类精子暴露于一种识别精子顶体蛋白酶的单克隆抗体（C11H）。在精子获能和/或授精过程中加入该抗体，并使用去透明带仓鼠卵测试其对穿透的影响。当授精时存在抗体时观察到对穿透有抑制作用，但仅在获能培养基中加入抗体时则没有这种作用。通过免疫荧光显微镜判断，大多数与卵表面结合的精子没有顶体染色。一些结合的精子在其赤道段有染色。穿透卵质的精子没有显示免疫荧光。
