Clemente Antonio, Alba-Patiño Alejandra, Rojo-Molinero Estrella, Russell Steven M, Borges Marcio, Oliver Antonio, de la Rica Roberto
Multidisciplinary Sepsis Group, Health Research Institute of the Balearic Islands (IdISBa), 07120 Palma de Mallorca, Spain.
Servicio de Microbiología, Hospital Son Espases, Health Research Institute of the Balearic Islands (IdISBa), 07120 Palma de Mallorca, Spain.
ACS Sens. 2020 Dec 24;5(12):3956-3963. doi: 10.1021/acssensors.0c01618. Epub 2020 Nov 24.
Respiratory infections caused by multi-drug-resistant often yield poor outcomes if not detected right away. However, detecting this pathogen in respiratory samples with a rapid diagnostic test is challenging because the protective biofilms created by the pathogen are themselves surrounded by a high-viscosity sputum matrix. Here, we introduce a method for liquefying respiratory samples and disrupting bacterial biofilms on the spot within a minute. It relies on the generation of oxygen bubbles by bacterial catalase through the addition of hydrogen peroxide. When coupled with a mobile biosensor made of paper, the resulting diagnostic kit was able to detect infections in sputa from patients with excellent sensitivity and specificity within 8 min. The quick turnaround time along with few infrastructure requirements make this method ideal for the rapid screening of infections at the point of care.
由多重耐药菌引起的呼吸道感染如果不能立即被检测到，往往会产生不良后果。然而，使用快速诊断测试在呼吸道样本中检测这种病原体具有挑战性，因为病原体形成的保护性生物膜本身被高粘度的痰液基质所包围。在这里，我们介绍一种在一分钟内就地液化呼吸道样本并破坏细菌生物膜的方法。它依赖于通过添加过氧化氢，由细菌过氧化氢酶产生氧气气泡。当与纸质移动生物传感器结合使用时，由此产生的诊断试剂盒能够在8分钟内以优异的灵敏度和特异性检测患者痰液中的感染。快速的周转时间以及极少的基础设施要求使这种方法成为即时护理点快速筛查感染的理想选择。
