Programa de Pós-graduação em Genética e Bioquímica, Instituto de Biotecnologia, Universidade Federal de Uberlândia (UFU), Uberlândia, MG 38408-100, Brazil.
Departamento de Ciências Fisiológicas, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia (UFU), Uberlândia, MG 38408-100, Brazil.
J Nat Prod. 2020 Dec 24;83(12):3698-3705. doi: 10.1021/acs.jnatprod.0c00958. Epub 2020 Nov 24.
(Winteraceae) has been investigated in traditional medicine for its anti-inflammatory properties to treat gastric ulcers and allergic and respiratory system diseases as well as for cancer treatment. In this work, we investigate the ability of the sesquiterpene polygodial, isolated from stem barks, to modulate the chronic inflammatory response induced by polyester-polyurethane sponge implants in C57BL/6J mice. Daily treatment with polygodial inhibited the macrophage content in the implants as determined by the activity of the -acetyl-β-d-glucosaminidase enzyme as well as decreased the levels of CXCL1/KC and CCL2/JE/MCP-1 pro-inflammatory chemokines and the presence of mast cells along the formed fibrovascular tissue. Similarly, the deposition of a new extracellular matrix (total collagen and type I and III collagen fibers) as well as the production of the TGF-β1 cytokine were attenuated in implants treated with polygodial, showing for the first time its antifibrogenic capacity. The hemoglobin content, the number of newly formed vessels, and the levels of VEGF cytokine, which were used as parameters for the assessment of the neovascularization of the implants, did not change after treatment with polygodial. The anti-inflammatory and antifibrogenic effects of polygodial over the components of the granulation tissue induced by the sponge implant indicate a therapeutic potential for the treatment of inflammatory diseases associated with the development of fibrovascular tissue.
（Winteraceae）因其具有抗炎特性而在传统医学中得到研究，可用于治疗胃溃疡以及过敏和呼吸系统疾病，还可用于癌症治疗。在这项工作中，我们研究了从 茎皮中分离出的倍半萜聚甲酚醛，调节聚酯-聚氨基甲酸酯海绵植入物在 C57BL/6J 小鼠中引起的慢性炎症反应的能力。聚甲酚醛的每日治疗通过 -乙酰基-β-d-葡糖苷酸酶的活性抑制了植入物中的巨噬细胞含量，同时降低了 CXCL1/KC 和 CCL2/JE/MCP-1 促炎趋化因子的水平以及形成的纤维血管组织中肥大细胞的存在。同样，在用聚甲酚醛处理的植入物中，新细胞外基质（总胶原蛋白和 I 型和 III 型胶原蛋白纤维）的沉积以及 TGF-β1 细胞因子的产生也减弱，这首次显示出其抗纤维化能力。用作植入物新生血管化评估参数的血红蛋白含量、新形成血管的数量以及 VEGF 细胞因子的水平在聚甲酚醛治疗后没有改变。聚甲酚醛对海绵植入物诱导的肉芽组织成分的抗炎和抗纤维化作用表明，其具有治疗与纤维血管组织形成相关的炎症性疾病的潜力。
