Komarova Natalia, Barkova Daria, Kuznetsov Alexander
Scientific-Manufacturing Complex Technological Centre, 1-7 Shokin Square, Zelenograd, 124498 Moscow, Russia.
Int J Mol Sci. 2020 Nov 20;21(22):8774. doi: 10.3390/ijms21228774.
Aptamers are nucleic acid ligands that bind specifically to a target of interest. Aptamers have gained in popularity due to their high potential for different applications in analysis, diagnostics, and therapeutics. The procedure called systematic evolution of ligands by exponential enrichment (SELEX) is used for aptamer isolation from large nucleic acid combinatorial libraries. The huge number of unique sequences implemented in the in vitro evolution in the SELEX process imposes the necessity of performing extensive sequencing of the selected nucleic acid pools. High-throughput sequencing (HTS) meets this demand of SELEX. Analysis of the data obtained from sequencing of the libraries produced during and after aptamer isolation provides an informative basis for precise aptamer identification and for examining the structure and function of nucleic acid ligands. This review discusses the technical aspects and the potential of the integration of HTS with SELEX.
适配体是能特异性结合目标物的核酸配体。由于适配体在分析、诊断和治疗等不同应用方面具有很高的潜力，因而越来越受到人们的欢迎。通过指数富集进行配体的系统进化（SELEX）这一程序被用于从大型核酸组合文库中分离适配体。SELEX过程中的体外进化所采用的大量独特序列使得对所选核酸库进行广泛测序成为必要。高通量测序（HTS）满足了SELEX的这一需求。对适配体分离过程中和分离后产生的文库测序所获得的数据进行分析，为精确鉴定适配体以及研究核酸配体的结构和功能提供了信息丰富的基础。本综述讨论了高通量测序与SELEX整合的技术方面及潜力。
