Payton Laura, Noirot Céline, Hoede Claire, Hüppe Lukas, Last Kim, Wilcockson David, Ershova Elizaveta A, Valière Sophie, Meyer Bettina
Institute for Chemistry and Biology of the Marine Environment, Carl von Ossietzky University of Oldenburg, Oldenburg, 26111, Germany.
Section Polar Biological Oceanography, Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research, Bremerhaven, 27570, Germany.
Sci Data. 2020 Nov 24;7(1):415. doi: 10.1038/s41597-020-00751-4.
The zooplankter Calanus finmarchicus is a member of the so-called "Calanus Complex", a group of copepods that constitutes a key element of the Arctic polar marine ecosystem, providing a crucial link between primary production and higher trophic levels. Climate change induces the shift of C. finmarchicus to higher latitudes with currently unknown impacts on its endogenous timing. Here we generated a daily transcriptome of C. finmarchicus at two high Arctic stations, during the more extreme time of Midnight Sun, the summer solstice. While the southern station (74.5 °N) was sea ice-free, the northern one (82.5 °N) was sea ice-covered. The mRNAs of the 42 samples have been sequenced with an average of 126 ± 5 million reads (mean ± SE) per sample, and aligned to the reference transcriptome. We detail the quality assessment of the datasets and the complete annotation procedure, providing the possibility to investigate daily gene expression of this ecologically important species at high Arctic latitudes, and to compare gene expression according to latitude and sea ice-coverage.
浮游动物飞马哲水蚤是所谓“哲水蚤复合体”的成员之一，该复合体是一群桡足类动物，是北极极地海洋生态系统的关键要素，在初级生产和更高营养级之间提供了至关重要的联系。气候变化导致飞马哲水蚤向更高纬度转移，目前对其内源生物钟的影响尚不清楚。在此，我们在北极地区两个站点，在极昼这一更为极端的时间，即夏至期间，生成了飞马哲水蚤的每日转录组。南部站点（北纬74.5°）无海冰覆盖，而北部站点（北纬82.5°）则被海冰覆盖。对42个样本的mRNA进行了测序，每个样本平均有1.26±0.05亿条读数（平均值±标准误），并与参考转录组进行比对。我们详细介绍了数据集的质量评估和完整的注释程序，可以在北极高纬度地区研究这种具有生态重要性的物种的每日基因表达情况，并根据纬度和海冰覆盖情况比较基因表达。
