Ni Zhonglin, Xing Dong, Zhang Teming, Ding Ning, Xiang Dan, Zhao Zhiguang, Qu Jinmiao, Hu Changyuan, Shen Xian, Xue Xiangyang, Zhou Jie
Division of Hepatobiliopancreatic Surgery, Department of General Surgery, Nanfang Hospital, Southern Medical University, No. 1838 North Guangzhou Avenue, Guangzhou, 510515, Guangdong Province, China.
Department of Microbiology and Immunology, Institute of Molecular Virology and Immunology, School of Basic Medical Sciences, Wenzhou Medical University, University Town, Chashan, Wenzhou, 325035, Zhejiang Province, China.
Immunol Res. 2021 Feb;69(1):43-52. doi: 10.1007/s12026-020-09167-z. Epub 2020 Nov 24.
This study aimed to further explore the clinicopathological correlation of B cell infiltration in gastric cancer (GC) and its impact on prognostic. By immunohistochemical method, CD20+ B cells, CD3+ T cells, CD66b+ tumor-associated neutrophils, CD163+ tumor-associated macrophages, and CD57+ natural killer cells were analyzed in consecutive sections of 584 GC tissues and 69 normal adjacent tissues. Kaplan-Meier and Cox regression analyses determined the relationship between clinical relevance or prognosis and B cell infiltration. The correlation between total B cell infiltration and various T cell subtype infiltration in GC tissues from 407 patients in the TCGA data was also analyzed. Kaplan-Meier and Cox regression analyses determined the effects of total B cell infiltration and various B cell subtype infiltration on the prognosis of patients with GC. The infiltration level of CD20+ B cells was positively correlated with that of T cells (risk ratio [RR] = 0.0930), especially CD4+ T cells and CD8+ T cells (P < 0.05). A high level of CD20+ B cell infiltration was significantly associated with low lymph node involvement and low TNM stage (P < 0.05). High levels of CD20+ B cell infiltration were significantly associated with improvements in overall survival and disease-free survival. Univariate Cox regression and multivariate Cox regression analysis showed that CD20+ B cell infiltration was an independent protective factor of prognosis. Higher levels of class-switched memory B cell and plasma cell also reflected better overall survival, and class-switched memory B cell and plasma cell were independent protective factors for prognosis. The findings indicate that B cell infiltration in GC, especially switched memory B cells and plasma cells, has a significant effect on tumor progression and prognosis.
本研究旨在进一步探讨胃癌（GC）中B细胞浸润的临床病理相关性及其对预后的影响。采用免疫组织化学方法，对584例GC组织和69例正常相邻组织的连续切片进行分析，检测CD20+B细胞、CD3+T细胞、CD66b+肿瘤相关中性粒细胞、CD163+肿瘤相关巨噬细胞和CD57+自然杀伤细胞。采用Kaplan-Meier法和Cox回归分析确定临床相关性或预后与B细胞浸润之间的关系。同时分析了TCGA数据中407例患者GC组织中总B细胞浸润与各种T细胞亚型浸润之间的相关性。采用Kaplan-Meier法和Cox回归分析确定总B细胞浸润和各种B细胞亚型浸润对GC患者预后的影响。CD20+B细胞浸润水平与T细胞浸润水平呈正相关（风险比[RR]= 0.0930），尤其是CD4+T细胞和CD8+T细胞（P < 0.05）。CD20+B细胞浸润水平高与低淋巴结转移和低TNM分期显著相关（P < 0.05）。CD20+B细胞浸润水平高与总生存期和无病生存期的改善显著相关。单因素Cox回归和多因素Cox回归分析显示，CD2+B细胞浸润是预后的独立保护因素。类别转换记忆B细胞和浆细胞水平较高也反映了较好的总生存期，类别转换记忆B细胞和浆细胞是预后的独立保护因素。研究结果表明，GC中的B细胞浸润，尤其是类别转换记忆B细胞和浆细胞，对肿瘤进展和预后有显著影响。
