Department of Gastrointestinal Surgery, Harbin Medical University Cancer Hospital, Harbin Medical University, Harbin, Heilongjiang 150086, China.
Department of Pathology, Harbin Medical University, Harbin, Heilongjiang 150001, China.
J Immunol Res. 2021 Feb 28;2021:6539702. doi: 10.1155/2021/6539702. eCollection 2021.
Serum CD4, CD8, and CD19 are markers of systemic inflammation. However, there is little evidence on the influence of inflammation on the tumor microenvironment and the prognostic indicators of gastric cancer (GC). In this study, two hundred and eight patients who underwent radical gastrectomy for GC were included. Preoperative peripheral blood samples were used to analyze Serum CD4, CD8, and CD19. The optimal cutoff levels for CD4, CD8, and CD19 were defined by receiver operating characteristic curve analysis (CD4 = 38.85%, CD8 = 14.35%, and CD19 = 7.40%). The areas with specific CD4T cells, CD8T cells, and CD19B cells within the tumor microenvironment were measured in paraffin sections by immunohistochemistry and analyzed by Image-Pro Plus. 94 patients had low CD4, and 124 patients had high CD4 levels. 31 patients had low CD8, and 187 patients had high CD8 levels. 64 patients had low CD19, and 154 patients had high CD19 levels. Infiltration of CD4T cells was associated with serum CD4 ( < 0.001). Serum CD4 and CD19 and the infiltration of CD4T cells, CD8T cells, and CD19B cells were significant in predicting the prognosis of GC. Low CD4 level, infiltration of CD8T cells, and high infiltration of CD4T cells and CD19B cells were correlated with worse overall survival in multivariate analysis. Collectively, our results provide evidence that serum CD4 is associated with the infiltration of CD4T cells in the tumor microenvironment, which indicates the prognostic value of systemic inflammation in GC.
血清 CD4、CD8 和 CD19 是全身炎症的标志物。然而，关于炎症对胃癌（GC）肿瘤微环境和预后指标的影响的证据很少。在这项研究中，纳入了 208 例接受 GC 根治性胃切除术的患者。使用术前外周血样分析血清 CD4、CD8 和 CD19。通过接收者操作特征曲线分析（CD4=38.85%，CD8=14.35%，CD19=7.40%）定义 CD4、CD8 和 CD19 的最佳截断值。通过免疫组织化学在石蜡切片中测量肿瘤微环境中特定 CD4T 细胞、CD8T 细胞和 CD19B 细胞的面积，并通过 Image-Pro Plus 进行分析。94 例患者 CD4 水平低，124 例患者 CD4 水平高。31 例患者 CD8 水平低，187 例患者 CD8 水平高。64 例患者 CD19 水平低，154 例患者 CD19 水平高。CD4T 细胞浸润与血清 CD4 相关（<0.001）。血清 CD4 和 CD19 以及 CD4T 细胞、CD8T 细胞和 CD19B 细胞的浸润对 GC 预后有显著预测作用。多因素分析显示，低 CD4 水平、CD8T 细胞浸润和高 CD4T 细胞和 CD19B 细胞浸润与总生存期较差相关。综上所述，我们的研究结果提供了证据表明，血清 CD4 与肿瘤微环境中 CD4T 细胞的浸润有关，这表明全身炎症在 GC 中的预后价值。
