Cho Won-Woo, Kim Byoung Soo, Ahn Minjun, Ryu Yeon Hee, Ha Dong-Heon, Kong Jeong Sik, Rhie Jong-Won, Cho Dong-Woo
Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), 77 Cheongam-ro, Nam-gu, Pohang, Kyungbuk, 37673, Republic of Korea.
POSTECH-Catholic Biomedical Engineering Institute, POSTECH, Pohang, Kyungbuk, 37673, Republic of Korea.
Adv Healthc Mater. 2021 Mar;10(6):e2001693. doi: 10.1002/adhm.202001693. Epub 2020 Nov 25.
A new concept, assembling cell-laden tissue modules, is for the first time proposed for soft tissue engineering. Adipose-vascular tissue modules composed of a synthetic polymer-based substructure and customized bioinks using planar 3D cell printing are engineered. Such tissue modules are systematically assembled into a synthetic polymer-based module holder fabricated with rotational 3D printing, resulting in the development of a flexible and volumetric tissue assembly. Whereas most of the previous studies about the construction of adipose tissue are limited to hypoxia, poor vascularization, rapid resorption, and mismatch in mechanical properties, it is aimed to realize the construction of nonhypoxic, flexible, and volume-stable tissue assembly in this study. The significance of engineered tissue assembly is proven through various in vitro and in vivo evaluations. In particular, stable volume and remarkable neovascularization/adipogenesis are observed in the implanted assembly over four weeks. Interestingly, the size of newly formed lipid droplets and the remodeled morphology in the assembly are comparable to those in native adipose tissue. As far as it is known, this work is a first report suggesting a cell printing-based tissue assembly for functional reconstruction of soft tissue.
一种用于软组织工程的新概念——组装载细胞组织模块，首次被提出。利用平面3D细胞打印技术，构建了由合成聚合物基子结构和定制生物墨水组成的脂肪 - 血管组织模块。这些组织模块被系统地组装到一个通过旋转3D打印制造的合成聚合物基模块支架中，从而形成了一种灵活的、具有一定体积的组织组件。以往关于脂肪组织构建的大多数研究都局限于缺氧、血管化不良、快速吸收以及机械性能不匹配等问题，而本研究旨在实现构建非缺氧、灵活且体积稳定的组织组件。通过各种体外和体内评估，证明了工程化组织组件的重要性。特别是，在植入的组件中观察到四周内体积稳定且有显著的新血管形成/脂肪生成。有趣的是，组件中新形成的脂滴大小和重塑的形态与天然脂肪组织中的相当。据所知，这项工作是首次报道基于细胞打印的组织组件用于软组织功能重建。
