Biomaterials Research Center, Korea Institute of Science and Technology (KIST), 5, Hwarang-ro 14-gil, Seongbuk-gu, Seoul 02792, Korea.
R&D Center, Medifab Co., Ltd., 70 Dusan-ro, Geumcheon-gu, Seoul 08584, Korea.
Int J Mol Sci. 2021 Nov 22;22(22):12560. doi: 10.3390/ijms222212560.
A dome-shaped elastic poly(l-lactide--caprolactone) (PLCL) scaffold with a channel and pore structure was fabricated by a combinative method of 3D printing technology and the gel pressing method (13 mm in diameter and 6.5 mm in thickness) for patient-specific regeneration. The PLCL scaffold was combined with adipose decellularized extracellular matrix (adECM) and heart decellularized extracellular matrix (hdECM) hydrogels and human adipose-derived stem cells (hADSCs) to promote adipogenesis and angiogenesis. These scaffolds had mechanical properties similar to those of native adipose tissue for improved tissue regeneration. The results of the in vitro real-time PCR showed that the dECM hydrogel mixture induces adipogenesis. In addition, the in vivo study at 12 weeks demonstrated that the tissue-engineered PLCL scaffolds containing the hydrogel mixture (hdECM/adECM (80:20)) and hADSCs promoted angiogenesis and adipose tissue formation, and suppressed apoptosis. Therefore, we expect that our constructs will be clinically applicable as material for the regeneration of patient-specific large-sized adipose tissue.
一种具有通道和孔结构的圆顶形弹性聚（L-丙交酯-己内酯）（PLCL）支架，通过 3D 打印技术和凝胶压制方法（直径 13mm，厚度 6.5mm）制造，用于特定于患者的再生。PLCL 支架与脂肪脱细胞细胞外基质（adECM）和心脏脱细胞细胞外基质（hdECM）水凝胶和人脂肪源性干细胞（hADSCs）结合，促进脂肪生成和血管生成。这些支架具有类似于天然脂肪组织的机械性能，可促进组织再生。体外实时 PCR 的结果表明，dECM 水凝胶混合物可诱导脂肪生成。此外，12 周的体内研究表明，含有水凝胶混合物（hdECM/adECM（80:20））和 hADSCs 的组织工程 PLCL 支架促进了血管生成和脂肪组织形成，并抑制了细胞凋亡。因此，我们期望我们的构建物将作为用于再生特定于患者的大型脂肪组织的材料在临床上得到应用。
