Alves Melina C C, Chaves Douglas S A, Benevenuto Byanca R, Farias Beatriz O DE, Coelho Shana M O, Ferreira Thais P, Pereira Geraldo A, Santos Gabriela C M Dos, Moreira Leandra O, Freitas Juliana P DE, Cid Yara P
Programa de Pós-Graduação em Ciências Veterinárias, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, BR-465, Km 7, 23897-000 Seropédica, RJ, Brazil.
Departamento de Ciências Farmacêuticas, Instituto de Ciências Biológicas e da Saúde, Universidade Federal Rural do Rio de Janeiro, BR-465, Km 7, 23897-000 Seropédica, RJ, Brazil.
An Acad Bras Cienc. 2020 Nov 20;92(4):e20200562. doi: 10.1590/0001-3765202020200562. eCollection 2020.
Periodontal disease is considered the main oral cavity disorder in dogs. Essential oils have the potential for use in the prevention and treatment of oral diseases. The antimicrobial activity of Schinus molle L. essential oil (SMEO) has already been reported. Chitosan, a natural product with antimicrobial activity and good biocompatibility has potential in biodental applications. In this study, we evaluated the in vitro antimicrobial activity of SMEO against bacteria associated with periodontal disease in dogs, developed and evaluated the physicochemical properties of a novel chitosan-based buccal delivery system containing SMEO. SMEO showed antimicrobial activity against Gram positive and Gram negative bacteria associated with canine periodontitis, with MIC values of 750 µg.mL-1 for Staphylococcus spp. and Streptococcus spp, 1000 µg.mL-1 for Corynebacterium spp. and 1250 µg.mL-1 for Pseudomonas spp. All formulations evaluated presented adequate physicochemical properties, good stability, and pH values close to buccal pH (5.0-7.0). Chitosan gel loaded with SMEO showed potential as a SMEO delivery system, having the ideal physicochemical and rheological properties (pseudoplastic and apparent viscosities) required for application on buccal tissue. Thus, we can conclude that formulation has the potential to be used for buccal mucosa delivery in the prevention and treatment of periodontal disease in dogs.
牙周病被认为是犬类主要的口腔疾病。精油有用于预防和治疗口腔疾病的潜力。已报道了秘鲁胡椒树精油（SMEO）的抗菌活性。壳聚糖是一种具有抗菌活性且生物相容性良好的天然产物，在生物牙科应用中有潜力。在本研究中，我们评估了SMEO对与犬牙周病相关细菌的体外抗菌活性，开发并评估了一种含SMEO的新型壳聚糖基口腔给药系统的物理化学性质。SMEO对与犬牙周炎相关的革兰氏阳性菌和革兰氏阴性菌均表现出抗菌活性，其中对葡萄球菌属和链球菌属的最低抑菌浓度（MIC）值为750 µg.mL-1，对棒状杆菌属为1000 µg.mL-1，对假单胞菌属为1250 µg.mL-1。所有评估的制剂均呈现出足够的物理化学性质、良好的稳定性，且pH值接近口腔pH（5.0 - 7.0）。负载SMEO的壳聚糖凝胶显示出作为SMEO给药系统的潜力，具有应用于口腔组织所需的理想物理化学和流变学性质（假塑性和表观粘度）。因此，我们可以得出结论，该制剂有潜力用于犬牙周病预防和治疗的口腔黏膜给药。
