The Wistar Institute, Philadelphia, PA, USA.
Methods Mol Biol. 2021;2236:19-28. doi: 10.1007/978-1-0716-1060-2_3.
Myeloid-derived suppressor cells (MDSC) are immunosuppressive myeloid cells that accumulate in tumor sites and peripheral lymphoid organs such as the spleen. In murine cancer models, the spleen is a major reservoir for MDSC, representing an easily accessible tissue from which to isolate high numbers of these cell population for downstream applications. Here we describe an efficient method to phenotype as well as to isolate and assess the functionality of murine splenic MDSC.
髓系来源的抑制性细胞（MDSC）是一种免疫抑制性的髓系细胞，在肿瘤部位和外周淋巴器官（如脾脏）中积聚。在小鼠肿瘤模型中，脾脏是 MDSC 的主要储存库，是一种容易获取的组织，可以从中分离出大量的这种细胞群体，用于下游应用。在这里，我们描述了一种有效鉴定以及分离和评估小鼠脾脏 MDSC 功能的方法。
