Division of Molecular Biology and Human Genetics, Faculty of Medical and Health Sciences, DST-NRF Centre of Excellence for Biomedical Tuberculosis Research, South African Medical Research Council for Tuberculosis Research, Stellenbosch University, Cape Town, South Africa.
Methods Mol Biol. 2021;2236:129-156. doi: 10.1007/978-1-0716-1060-2_12.
The current absence of markers unique to MDSC, particularly those expanded during human infection, necessitate concurrent demonstration of their suppressive capacity to ensure unequivocal identification. This is further complicated by the array of heterogeneous markers used to characterize MDSC in various conditions and models. Standardization of phenotypic and functional characterization, as well as isolation, from infectious biological samples of patients, are critical for accurately reporting MDSC dynamics, function, organ abundance, and establishment of their therapeutic value in infectious diseases. To illustrate, we report on our established method for MDSC isolation from bronchoalveolar lavage fluid and peripheral blood of pulmonary TB patients, as well as functional impact on T cells by measuring T cell activation, proliferation, and cytokine production.
目前缺乏 MDSC 特有的标志物，特别是在人类感染期间扩增的标志物，因此需要同时证明其抑制能力，以确保明确的鉴定。这进一步复杂化了用于在各种情况下和模型中表征 MDSC 的异质标志物的多样性。对来自感染性生物样本的患者进行表型和功能特征的标准化以及分离，对于准确报告 MDSC 的动态、功能、器官丰度以及在传染病中建立其治疗价值至关重要。例如，我们报告了从肺结核患者支气管肺泡灌洗液和外周血中分离 MDSC 的既定方法，以及通过测量 T 细胞活化、增殖和细胞因子产生来评估对 T 细胞的功能影响。
