Department of Medical Biology, Faculty of Medicine, Afyonkarahisar Health Sciences University, Afyonkarahisar, Turkey
Department of Medical Genetic, Faculty of Medicine, Afyonkarahisar Health Sciences University, Afyonkarahisar, Turkey
Turk J Med Sci. 2021 Apr 30;51(2):661-674. doi: 10.3906/sag-2010-18.
BACKGROUND/AIM: The aim of the study is to assess expression levels of CPEB4, APC, TRIP13, EIF2S3, EIF4A1, IFNg, PIK3CA and CTNNB1 genes in tumors and peripheral bloods of colorectal cancer patients in stages I–IV.
The mRNA levels of the genes were determined in tumor tissues and peripheral blood samples of 45 colorectal cancer patients and colon tissues and peripheral blood samples of 5 healthy individuals. Real-time polymerase chain reaction method was used for the analysis.
The mRNA level of the CPEB4 gene was significantly downregulated in colorectal tumor tissues and was upregulated in the peripheral blood of colorectal cancer patients relative to the controls (P < 0.05). APC mRNA level was significantly downregulated in tissues and upregulated in the peripheral blood (P < 0.05). TRIP13 mRNA level was upregulated in peripheral blood and also significantly upregulated in colorectal tumor tissues (P < 0.05). EIF2S3 mRNA level was upregulated in tissues and also significantly upregulated in peripheral blood (P < 0.05). PIK3CA mRNA level was downregulated in tissues and upregulated in peripheral blood. EIF4A1 mRNA level was downregulated in tissues and significantly upregulated in peripheral blood (P < 0.05). CTNNB1 mRNA level was downregulated in tissues and upregulated in peripheral blood. IFNg mRNA level was upregulated in both colorectal cancer tumor tissues and peripheral blood.
TRIP13 and CPEB4 mRNA up regulation in the peripheral blood of patients with colorectal cancer may be a potential target for early stage diagnosis. In addition to this evaluation, although there is not much study on EIF2S3 and EIF4A1 mRNA changes in cases with colorectal cancer, upregulation in peripheral blood draws attention in our study. These data will shed light on the new comprehensive studies.
背景/目的：本研究旨在评估 CPEB4、APC、TRIP13、EIF2S3、EIF4A1、IFNg、PIK3CA 和 CTNNB1 基因在 I-IV 期结直肠癌患者肿瘤和外周血中的表达水平。
采用实时聚合酶链反应法检测 45 例结直肠癌患者肿瘤组织和外周血样本及 5 例健康对照者结肠组织和外周血样本中基因的 mRNA 水平。
CPEB4 基因在结直肠肿瘤组织中的 mRNA 水平显著下调，而在结直肠癌患者的外周血中上调（P<0.05）。APC mRNA 水平在组织中显著下调，在外周血中上调（P<0.05）。TRIP13 mRNA 水平在外周血中上调，在结直肠肿瘤组织中也显著上调（P<0.05）。EIF2S3 mRNA 水平在组织中上调，在外周血中也显著上调（P<0.05）。PIK3CA mRNA 水平在组织中下调，在外周血中上调。EIF4A1 mRNA 水平在组织中下调，在外周血中显著上调（P<0.05）。CTNNB1 mRNA 水平在组织中下调，在外周血中上调。IFNg mRNA 水平在结直肠癌肿瘤组织和外周血中均上调。
结直肠癌患者外周血中 TRIP13 和 CPEB4 mRNA 的上调可能是早期诊断的潜在靶点。除了这种评估，尽管我们的研究中 EIF2S3 和 EIF4A1 mRNA 变化在结直肠癌病例中研究较少，但在外周血中的上调引起了我们的注意。这些数据将为新的综合研究提供启示。
