Ihua Maureen W, FitzGerald Jamie A, Guihéneuf Freddy, Jackson Stephen A, Claesson Marcus J, Stengel Dagmar B, Dobson Alan D W
School of Microbiology, University College Cork, Cork, Ireland.
APC Microbiome Institute, University College Cork, Cork, Ireland.
PLoS One. 2020 Nov 25;15(11):e0242675. doi: 10.1371/journal.pone.0242675. eCollection 2020.
Stipitate kelp species such as Laminaria digitata dominate most cold-water subtidal rocky shores and form underwater forests which are among the most productive coastal systems worldwide. Laminaria also sustains rich bacterial communities which offer a variety of biotechnological applications. However, to date, in-depth studies on the diversity and uniqueness of bacterial communities associated with this macroalgal species, their ecological role and their interactions with the alga are under-represented. To address this, the epibacterial populations associated with different thallus regions (holdfast, stipe, meristem, blade) of this brown seaweed were investigated using high-throughput Illumina sequencing of the 16S rRNA genes. The results show that epibacterial communities of the brown seaweed are significantly different and specific to the thallus region, with the shared bacterial population comprising of only 1.1% of the total amplicon sequence variants. The diverse holdfast and blade tissues formed distinct clusters while the meristem and stipe regions are more closely related. The data obtained further supports the hypothesis that macroalgal bacterial communities are shaped by morphological niches and display specificity.
像掌状海带这样的柄状海带物种在大多数冷水潮下带岩石海岸占主导地位，并形成水下森林，这些水下森林是全球生产力最高的沿海系统之一。海带还维持着丰富的细菌群落，这些细菌群落具有多种生物技术应用。然而，迄今为止，关于与这种大型藻类物种相关的细菌群落的多样性和独特性、它们的生态作用以及它们与藻类的相互作用的深入研究还很少。为了解决这个问题，利用16S rRNA基因的高通量Illumina测序技术，对这种褐藻不同藻体区域（固着器、柄、分生组织、叶片）相关的附生细菌种群进行了研究。结果表明，褐藻的附生细菌群落存在显著差异，并且对藻体区域具有特异性，共有细菌种群仅占总扩增子序列变体的1.1%。多样的固着器和叶片组织形成了不同的聚类，而分生组织和柄区域的关系更为密切。获得的数据进一步支持了大型藻类细菌群落受形态生态位影响并具有特异性的假说。
