5-氟乳清酸作为酵母分子遗传学中的一种选择剂。
5-Fluoroorotic acid as a selective agent in yeast molecular genetics.
作者信息
Boeke J D, Trueheart J, Natsoulis G, Fink G R
出版信息
Methods Enzymol. 1987;154:164-75. doi: 10.1016/0076-6879(87)54076-9.
PMID:3323810
Abstract
5-FOA is an extremely useful reagent for the selection of Ura- cells amid a population of Ura+ cells. The selection is effective in transformation and recombination studies where loss of URA3+ is desired. A new plasmid shuffling procedure based on the 5-FOAR selection permits the recovery of conditional lethal mutations in cloned genes that encode vital functions.
摘要
5-氟乳清酸（5-FOA）是一种极为有用的试剂，用于在尿嘧啶营养型（Ura+）细胞群体中筛选尿嘧啶缺陷型（Ura-）细胞。这种筛选在转化和重组研究中很有效，这些研究需要URA3+功能的缺失。基于5-FOA筛选的一种新的质粒洗牌程序能够在编码重要功能的克隆基因中获得条件致死突变。
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