Hantke K
Lehrstuhl für Mikrobiologie II, Universität Tübingen, Federal Republic of Germany.
Mol Gen Genet. 1987 Nov;210(1):135-9. doi: 10.1007/BF00337769.
A selection procedure using Mn2+ is described. A high percentage of the Mn2+ resistant mutants had constitutive iron transport systems. By P1 transduction, and complementation with the cloned fur gene it could be shown that nearly all the mutants constitutive in the expression of the operon fusion fiu::lambda placMu were only defective in fur. High concentrations of manganese inhibited the derepression of an iron-regulated lac operon fusion. In another iron-regulated lac operon fusion that was inducible by iron, manganese also induced the production of beta-galactosidase. Most of the fur mutants isolated (80%) were not able to grow on succinate, fumarate or acetate. After transformation with a fur+ plasmid all 39 mutants tested were able to grow on succinate. In fur mutants the presence of succinate in the growth medium reduced succinate uptake rates by 50%-70%. Succinate dehydrogenase activity was reduced to 10% of that of the parent strain.
描述了一种使用Mn2+的筛选方法。高比例的抗Mn2+突变体具有组成型铁转运系统。通过P1转导以及与克隆的fur基因互补，可以证明几乎所有在操纵子融合fiu::lambda placMu表达中组成型的突变体仅在fur基因上存在缺陷。高浓度的锰抑制了铁调节的lac操纵子融合的去阻遏。在另一个可被铁诱导的铁调节lac操纵子融合中，锰也诱导了β-半乳糖苷酶的产生。分离出的大多数fur突变体（80%）无法在琥珀酸盐、富马酸盐或乙酸盐上生长。用fur+质粒转化后，测试的所有39个突变体都能够在琥珀酸盐上生长。在fur突变体中，生长培养基中琥珀酸盐的存在使琥珀酸盐摄取率降低了50%-70%。琥珀酸脱氢酶活性降低至亲本菌株的10%。
