基因fadR在大肠杆菌乙酸代谢中的作用。
Role of gene fadR in Escherichia coli acetate metabolism.
作者信息
Maloy S R, Nunn W D
出版信息
J Bacteriol. 1981 Oct;148(1):83-90. doi: 10.1128/jb.148.1.83-90.1981.
Abstract
Mutants of Escherichia coli K-12 constitutive for fatty acid degradation (fadR) showed an increased rate of utilization of exogenous acetate. Acetate transport, oxidation, and incorporation into macromolecules was approximately fivefold greater in fadR mutants than fadR+ strains during growth on succinate as a carbon source. This effect was due to the elevated levels of glyoxylate shunt enzymes in fadR mutants, since (i) similar results were seen with mutants constitutive for the glyoxylate shunt enzymes (iclR), (ii) induction of the glyoxylate shunt in fadR+ strains by growth on acetate or oleate increased the rate of acetate utilization to levels comparable to those in fadR mutants, and (iii) fadR and fadR+ derivatives of mutants defective for the glyoxylate shunt enzymes showed equivalent rates of acetate utilization under these conditions. These results suggest that the operation of the glyoxylate shunt may play a significant role in the utilization of exogenous acetate by fadR mutants.
摘要
脂肪酸降解组成型的大肠杆菌K-12突变体（fadR）对外源乙酸盐的利用速率有所增加。在以琥珀酸盐作为碳源生长期间，fadR突变体中乙酸盐的转运、氧化以及掺入大分子的过程比fadR+菌株大约高五倍。这种效应是由于fadR突变体中乙醛酸循环酶水平升高所致，原因如下：（i）乙醛酸循环酶组成型的突变体（iclR）也观察到类似结果；（ii）fadR+菌株在乙酸盐或油酸盐上生长时乙醛酸循环的诱导使乙酸盐利用速率提高到与fadR突变体相当的水平；（iii）在这些条件下，乙醛酸循环酶缺陷的突变体的fadR和fadR+衍生物显示出相当的乙酸盐利用速率。这些结果表明，乙醛酸循环的运行可能在fadR突变体利用外源乙酸盐过程中起重要作用。
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