Jeju Inside Agency & Cosmetic Science Center, Jeju, Republic of Korea.
Department of Chemistry and Cosmetics, Jeju National University; Bio-Convergence Center, Jeju, Republic of Korea.
Pharmazie. 2020 Nov 1;75(11):565-570. doi: 10.1691/ph.2020.0522.
is an evergreen tree in the family , the seeds, leaves, and stems of which have long been used as edible products and herbal medicines in Korea. Previous studies of biological activity have shown that has antioxidant and anti-inflammatory effects. However, the effect of leaves on melanogenesis are yet to be studied. In this investigation, we used B16F10 melanoma cells to test the efficacy of leaf hot water extract (TLWE). α-melanocyte stimulating hormone (α-MSH) stimulated B16F10 melanoma cells were treated with various concentrations of TLWE (50, 100, and 200 μg/mL). The results showed that TLWE reduced the melanin content and cellular tyrosinase activity in a concentration-dependent manner. It also inhibited the phosphorylation of p38 mitogen-activated protein kinase (p38) and c-Jun N-terminal kinase (JNK) in the mitogen-activated protein kinase (MAPK) signaling pathway. The compounds catechin and ρ-coumaric acid, which are known to have a whitening effect on skin, were detected by HPLC analysis. These results suggest that TLWE has an anti-melanogenic effect. In addition, the safety of TLWE was tested. The results of the skin irritation test showed that TLWE is harmless to the human skin, even at higher concentrations than those used in the experiment. Therefore, we suggest that the water extract of leaves has potential for use as a skin-whitening agent.
是姜科姜黄属的常绿乔木，其种子、叶片和茎在韩国一直被作为食用产品和草药使用。以往对生物活性的研究表明，具有抗氧化和抗炎作用。然而，姜黄叶对黑色素生成的影响尚未被研究过。在本研究中，我们使用 B16F10 黑色素瘤细胞来测试姜黄热水提取物（TLWE）的功效。用不同浓度的 TLWE（50、100 和 200μg/ml）处理α-促黑素细胞激素（α-MSH）刺激的 B16F10 黑色素瘤细胞。结果表明，TLWE 以浓度依赖的方式降低黑色素含量和细胞酪氨酸酶活性。它还抑制丝裂原活化蛋白激酶（MAPK）信号通路中 p38 丝裂原活化蛋白激酶（p38）和 c-Jun N-末端激酶（JNK）的磷酸化。通过高效液相色谱分析检测到两种已知对皮肤有美白作用的化合物儿茶素和 ρ-香豆酸。这些结果表明 TLWE 具有抗黑色素生成作用。此外，还测试了 TLWE 的安全性。皮肤刺激性试验的结果表明，TLWE 对人体皮肤无害，即使在高于实验中使用的浓度下也是如此。因此，我们建议姜黄叶片的水提取物具有作为皮肤美白剂的潜力。
