Department of Stomatology, Renmin Hospital of Wuhan University, Wuhan.
Department of Pediatric Dentistry, The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University , Wuhan, China;, Email:
Pharmazie. 2020 Nov 1;75(11):576-580. doi: 10.1691/ph.2020.0652.
Gingival mesenchymal stem cells (GMSCs) have great potential in bone tissue regeneration. However, it is not well known how on exosomes derived from GMSCs affect the functions of bone-related cells. In this study, we explored the impact of GMSCs-derived exosomes (GMSCs-Exos) on pre-osteoblast MC3T3-E1 proliferation, migration and osteogenic differentiation. Results of CCK-8 assay showed that GMSCs-Exos had no effect on proliferation of pre-osteoblasts. Further, we found that GMSCs-Exos promoted the migration of pre-osteoblasts and osteogenic differentiation of MC3T3-E1 as revealed by enhanced Alizarin red staining, elevated alkaline phosphatase (ALP) activity and upregulated expression of osteogenic genes. This study provides new insights into the potential exosome-mediated paracrine mechanism of GMSCs in bone regeneration.
牙龈间充质干细胞（GMSCs）在骨组织再生中具有巨大的潜力。然而，目前尚不清楚 GMSCs 来源的外体如何影响与骨相关的细胞的功能。在这项研究中，我们探讨了 GMSCs 来源的外体（GMSCs-Exos）对成骨前体细胞 MC3T3-E1 增殖、迁移和成骨分化的影响。CCK-8 检测结果表明，GMSCs-Exos 对成骨前体细胞的增殖没有影响。进一步的研究发现，GMSCs-Exos 促进了成骨前体细胞的迁移和 MC3T3-E1 的成骨分化，这表现为茜素红染色增强、碱性磷酸酶（ALP）活性升高和成骨基因表达上调。本研究为 GMSCs 在骨再生中潜在的外体介导旁分泌机制提供了新的见解。
