Cátedra "B" de Química Biológica, Facultad de Odontología, Universidad Nacional de Córdoba (UNC), Córdoba, Argentina.
Instituto de Investigaciones en Ciencias de la Salud (INICSA/UNC-CONICET), Córdoba, Argentina.
Cell Biochem Funct. 2022 Aug;40(6):636-646. doi: 10.1002/cbf.3733. Epub 2022 Jul 18.
Melatonin (MEL) has antioxidant properties and participates in osteogenic differentiation. In periodontitis, in which increased oxidative stress and bone resorption are involved, mesenchymal stem cells derived from the gingiva (GMSCs) combined with MEL could be relevant for osteogenic regeneration. In this study, we studied the antioxidant and differentiating effect of MEL on an in vitro system of GMSCs. Primary culture of GMSCs from Wistar rats was developed to evaluate differentiation into osteoblasts with an appropriate medium with or without MEL. Marker genes of mesenchymal stem cells by real time-polymerase chain reaction, clonogenic capacity, and cell migration after wound assay were used to characterize GMSCs as mesenchymal stem cells. Alkaline phosphatase activity and the alizarin red technique were used to evaluate osteogenic activity and differentiation. MEL increased alkaline phosphatase activity and alizarin red values, promoting osteogenic differentiation. Besides this, MEL protected GMSCs in a model of cellular damage related to oxidative stress, returning viability to baseline. MEL was more effective in promoting and protecting GMSCs by the production of osteogenic cells when oxidative stress is present. This evidence supports the use of MEL as a novel bone-regenerative therapy in periodontal diseases.
褪黑素（MEL）具有抗氧化特性，并参与成骨分化。在牙周炎中，涉及氧化应激增加和骨吸收，牙龈来源的间充质干细胞（GMSCs）与 MEL 结合可能与成骨再生有关。在这项研究中，我们研究了 MEL 对 GMSCs 体外系统的抗氧化和分化作用。从 Wistar 大鼠中培养 GMSCs 的原代培养物，用于评估在适当的培养基中是否存在 MEL 向成骨细胞的分化。通过实时聚合酶链反应、集落形成能力和划痕试验后的细胞迁移来评估间充质干细胞的标志物基因，以将 GMSCs 鉴定为间充质干细胞。碱性磷酸酶活性和茜素红技术用于评估成骨活性和分化。MEL 增加碱性磷酸酶活性和茜素红值，促进成骨分化。除此之外，MEL 通过产生成骨细胞来保护 GMSCs 免受与氧化应激相关的细胞损伤，使细胞活力恢复到基线水平。当存在氧化应激时，MEL 更有效地促进和保护 GMSCs 生成成骨细胞。这一证据支持将 MEL 用作牙周病的新型骨再生治疗方法。
