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人牙龈间充质干细胞分泌的细胞外囊泡促进大鼠股骨骨缺损的骨再生。

Extracellular vesicles secreted by human gingival mesenchymal stem cells promote bone regeneration in rat femoral bone defects.

作者信息

Wang Situo, Liu Ziwei, Yang Shuo, Huo Na, Qiao Bo, Zhang Tong, Xu Juan, Shi Quan

机构信息

Department of Stomatology, The First Medical Center, Chinese PLA General Hospital, Beijing, China.

Orthopedic Laboratory of PLA General Hospital, Beijing, China.

出版信息

Front Bioeng Biotechnol. 2023 Feb 21;11:1098172. doi: 10.3389/fbioe.2023.1098172. eCollection 2023.

DOI:10.3389/fbioe.2023.1098172
PMID:36896013
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9989199/
Abstract

Extracellular vesicles (EVs), important components of paracrine secretion, are involved in various pathological and physiological processes of the body. In this study, we researched the benefits of EVs secreted by human gingival mesenchymal stem cells (hGMSC-derived EVs) in promoting bone regeneration, thereby providing new ideas for EVs-based bone regeneration therapy. Here, we successfully demonstrated that hGMSC-derived EVs could enhance the osteogenic ability of rat bone marrow mesenchymal stem cells and the angiogenic capability of human umbilical vein endothelial cells. Then, femoral defect rat models were created and treated with phosphate-buffered saline, nanohydroxyapatite/collagen (nHAC), a grouping of nHAC/hGMSCs, and a grouping of nHAC/EVs. The results of our study indicated that the combination of hGMSC-derived EVs and nHAC materials could significantly promote new bone formation and neovascularization with a similar effect to that of the nHAC/hGMSCs group. Our outcomes provide new messages on the role of hGMSC-derived EVs in tissue engineering, which exhibit great potential in bone regeneration treatment.

摘要

细胞外囊泡（EVs）是旁分泌分泌的重要组成部分，参与身体的各种病理和生理过程。在本研究中，我们研究了人牙龈间充质干细胞分泌的EVs（hGMSC衍生的EVs）在促进骨再生方面的益处，从而为基于EVs的骨再生治疗提供新思路。在此，我们成功证明hGMSC衍生的EVs可增强大鼠骨髓间充质干细胞的成骨能力和人脐静脉内皮细胞的血管生成能力。然后，建立股骨缺损大鼠模型，并用磷酸盐缓冲盐水、纳米羟基磷灰石/胶原蛋白（nHAC）、nHAC/hGMSCs组合以及nHAC/EVs组合进行治疗。我们的研究结果表明，hGMSC衍生的EVs与nHAC材料的组合可显著促进新骨形成和新血管形成，其效果与nHAC/hGMSCs组相似。我们的研究结果为hGMSC衍生的EVs在组织工程中的作用提供了新信息，其在骨再生治疗中具有巨大潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a08/9989199/b1ee59052d74/fbioe-11-1098172-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a08/9989199/c04e67e3c9ab/fbioe-11-1098172-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a08/9989199/f5015090a340/fbioe-11-1098172-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a08/9989199/605b8facac57/fbioe-11-1098172-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a08/9989199/352aee955261/fbioe-11-1098172-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a08/9989199/de3457188ff9/fbioe-11-1098172-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a08/9989199/3cf22be2a6cc/fbioe-11-1098172-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a08/9989199/9209cdf4b24a/fbioe-11-1098172-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a08/9989199/3d8c7507080b/fbioe-11-1098172-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a08/9989199/b1ee59052d74/fbioe-11-1098172-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a08/9989199/c04e67e3c9ab/fbioe-11-1098172-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a08/9989199/f5015090a340/fbioe-11-1098172-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a08/9989199/605b8facac57/fbioe-11-1098172-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a08/9989199/352aee955261/fbioe-11-1098172-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a08/9989199/de3457188ff9/fbioe-11-1098172-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a08/9989199/3cf22be2a6cc/fbioe-11-1098172-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a08/9989199/9209cdf4b24a/fbioe-11-1098172-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a08/9989199/3d8c7507080b/fbioe-11-1098172-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a08/9989199/b1ee59052d74/fbioe-11-1098172-g009.jpg

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