Liu Wei, Majumdar Sanghamitra, Li Weiwei, Keller Arturo A, Slaveykova Vera I
Department F.-A. Forel for Environmental and Aquatic Sciences, Environmental Biogeochemistry and Ecotoxicology, Faculty of Sciences, Earth and Environment Sciences, University of Geneva, Uni Carl Vogt,66 Blvd Carl-Vogt, 1211, Geneva, Switzerland.
Bren School of Environmental Science and Management, University of California, Santa Barbara, CA, 93106-5131, USA.
Sci Rep. 2020 Nov 25;10(1):20563. doi: 10.1038/s41598-020-77521-0.
Silver nanoparticles (AgNPs) are one of the most used engineered nanomaterials. Despite progress in assessing their environmental implications, knowledge gaps exist concerning the metabolic perturbations induced by AgNPs on phytoplankton, essential organisms in global biogeochemical cycles and food-web dynamics. We combine targeted metabolomics, biouptake and physiological response studies to elucidate metabolic perturbations in alga Poterioochromonas malhamensis induced by AgNPs and dissolved Ag. We show time-dependent perturbation of the metabolism of amino acids, nucleotides, fatty acids, tricarboxylic acids, photosynthesis and photorespiration by both Ag-treatments. The results suggest that dissolved Ag ions released by AgNPs are the major toxicity driver; however, AgNPs internalized in food vacuoles contributed to the perturbation of amino acid metabolism, TCA cycle and oxidative stress. The metabolic perturbations corroborate the observed physiological responses. We highlight the potential of metabolomics as a tool for understanding the molecular basis for these metabolic and physiological changes, and for early detection of stress.
银纳米颗粒（AgNPs）是使用最为广泛的工程纳米材料之一。尽管在评估其环境影响方面取得了进展，但在AgNPs对浮游植物（全球生物地球化学循环和食物网动态中的重要生物）诱导的代谢扰动方面仍存在知识空白。我们结合靶向代谢组学、生物摄取和生理反应研究，以阐明AgNPs和溶解态银对藻类马尔姆波氏色藻（Poterioochromonas malhamensis）诱导的代谢扰动。我们发现，两种银处理方式都会对氨基酸、核苷酸、脂肪酸、三羧酸、光合作用和光呼吸的代谢产生时间依赖性扰动。结果表明，AgNPs释放的溶解态银离子是主要的毒性驱动因素；然而，内化于食物泡中的AgNPs导致了氨基酸代谢、三羧酸循环和氧化应激的扰动。代谢扰动证实了所观察到的生理反应。我们强调了代谢组学作为一种工具的潜力，它有助于理解这些代谢和生理变化的分子基础，并用于早期应激检测。
