Lengeler Klaus B, Stovicek Vratislav, Fennessy Ross T, Katz Michael, Förster Jochen
Carlsberg Research Laboratory, Carlsberg A/S, Copenhagen, Denmark.
Front Genet. 2020 Nov 6;11:582789. doi: 10.3389/fgene.2020.582789. eCollection 2020.
Fermented foods and particularly beer have accompanied the development of human civilization for thousands of years. , the dominant yeast in the production of alcoholic beverages, probably co-evolved with human activity. Considering that alcoholic fermentations emerged worldwide, the number of strains used in beer production nowadays is surprisingly low. Thus, the genetic diversity is often limited. This is among others related to the switch from a household brewing style to a more artisan brewing regime during the sixteenth century and latterly the development of single yeast isolation techniques at the Carlsberg Research Laboratory in 1883, resulting in process optimizations in the brewing industry. However, due to fierce competition within the beer market and the increasing demand for novel beer styles, diversification is becoming increasingly important. Moreover, the emergence of craft brewing has influenced big breweries to rediscover yeast as a significant contributor to a beer's aroma profile and realize that there is still room for innovation in the fermentation process. Here, we aim at giving a brief overview on how currently used brewing yeasts emerged and comment on the rationale behind replacing them with novel strains. We will present potential sources of yeasts that have not only been used in beer brewing before, including natural sources and sources linked to human activity but also an overlooked source, such as yeast culture collections. We will briefly comment on common yeast isolation techniques and finally touch on additional challenges for the brewing industry in replacing their current brewer's yeasts.
发酵食品,尤其是啤酒,已经伴随人类文明发展了数千年。酒精饮料生产中的主要酵母,可能与人类活动共同进化。考虑到酒精发酵在全球范围内出现,如今用于啤酒生产的酵母菌株数量出奇地少。因此,遗传多样性往往有限。这尤其与16世纪家庭酿造方式向更具工匠风格酿造方式的转变以及1883年嘉士伯研究实验室单酵母分离技术的发展有关,这导致了酿造行业的工艺优化。然而,由于啤酒市场的激烈竞争以及对新型啤酒风格需求的增加,多样化变得越来越重要。此外,精酿啤酒的出现促使大型啤酒厂重新认识到酵母是啤酒香气特征的重要贡献者,并意识到发酵过程中仍有创新空间。在此,我们旨在简要概述当前使用的酿造酵母是如何出现的,并对用新型菌株取代它们背后的基本原理进行评论。我们将介绍酵母的潜在来源,这些来源不仅包括以前用于啤酒酿造的天然来源和与人类活动相关的来源,还包括一个被忽视的来源,如酵母菌种保藏中心。我们将简要介绍常见的酵母分离技术,最后探讨酿造行业在替换其当前啤酒酵母时面临的其他挑战。