Gupte Ameya Pankaj, Pierantoni Debora Casagrande, Conti Angela, Donati Leonardo, Basaglia Marina, Casella Sergio, Favaro Lorenzo, Corte Laura, Cardinali Gianluigi
Department of Agronomy Food natural Resources Animals and Environment (DAFNAE), University of Padova, 35020 Legnaro, Italy.
Department of Pharmaceutical Sciences, University of Perugia, 06121 Perugia, Italy.
J Fungi (Basel). 2023 Feb 16;9(2):264. doi: 10.3390/jof9020264.
Due to their long domestication time course, many industrial strains are adopted in numerous processes mostly for historical reasons instead of scientific and technological needs. As such, there is still significant room for improvement for industrial yeast strains relying on yeast biodiversity. This paper strives to regenerate biodiversity with the innovative application of classic genetic methods to already available yeast strains. Extensive sporulation was indeed applied to three different yeast strains, specifically selected for their different origins as well as backgrounds, with the aim of clarifying how new variability was generated. A novel and easy method to obtain mono-spore colonies was specifically developed, and, to reveal the extent of the generated variability, no selection after sporulation was introduced. The obtained progenies were then tested for their growth in defined mediums with high stressor levels. A considerable and strain-specific increase in both phenotypic and metabolomic variability was assessed, and a few mono-spore colonies were found to be of great interest for their future exploitation in selected industrial processes.
由于其漫长的驯化历程，许多工业菌株大多因历史原因而非科技需求被应用于众多工艺中。因此，依赖酵母生物多样性的工业酵母菌株仍有很大的改进空间。本文致力于通过将经典遗传方法创新性地应用于现有酵母菌株来恢复生物多样性。确实对三种不同的酵母菌株进行了广泛的孢子形成处理，这些菌株因其不同的来源和背景而被特别挑选，目的是阐明新的变异性是如何产生的。专门开发了一种新颖且简便的获取单孢子菌落的方法，并且，为了揭示所产生变异性的程度，在孢子形成后未进行选择。然后对获得的子代在含有高应激源水平的限定培养基中的生长情况进行测试。评估了表型和代谢组学变异性的显著且菌株特异性的增加，并且发现一些单孢子菌落在未来用于选定的工业过程中具有很大的开发价值。
