Liu Jia-Ying, Hu Yang, Li Long, Wang Chao, Wang Jia, Li Yang, Chen Dafu, Ding Xiaokang, Shen Chuanan, Xu Fu-Jian
State Key Laboratory of Chemical Resource Engineering Key Laboratory of Biomedical Materials of Natural Macromolecules (Beijing University of Chemical Technology, Ministry of Education) Beijing Laboratory of Biomedical Materials Beijing University of Chemical Technology Beijing 100029 China.
Laboratory of Bone Tissue Engineering Beijing Laboratory of Biomedical Materials Beijing Research Institute of Traumatology and Orthopaedics Beijing Jishuitan Hospital Beijing 100035 China.
Adv Sci (Weinh). 2020 Oct 4;7(22):2002243. doi: 10.1002/advs.202002243. eCollection 2020 Nov.
It is very desirable to develop advanced sustainable biomedical materials with superior biosafety and bioactivity for clinical applications. Herein, biomass-derived multilayer-structured absorbable microparticles (MQ T ) composed of starches and plant polyphenols are readily constructed for the safe and effective treatment of bone defects with intractable bleeding by coating multiple layers of quaternized starch (Q) and tannic acid onto microporous starch microparticles via facile layer-by-layer assembly. MQ T microparticles exhibit efficient degradability, low cytotoxicity, and good blood compatibility. Among various MQ T microparticles with distinct Q/T double layers, MQT with outmost polyphenol layer possess the unique properties of platelet adhesion/activation and red blood cell aggregation, resulting in the best hemostatic performance. In a mouse cancellous-bone-defect model, MQT exhibits the favorable hemostatic effect, low inflammation/immune responses, high biodegradability, and promoted bone repair. A proof-of-concept study of beagles further confirms the good performance of MQT in controlling intractable bleeding of bone defects. The present work demonstrates that such biomass-based multilayer-structured microparticles are very promising biomedical materials for clinical use.
开发具有卓越生物安全性和生物活性的先进可持续生物医学材料用于临床应用是非常可取的。在此，通过将多层季铵化淀粉（Q）和单宁酸通过简便的逐层组装涂覆在微孔淀粉微粒上，由淀粉和植物多酚组成的生物质衍生多层结构可吸收微粒（MQ T）很容易构建出来，用于安全有效地治疗伴有难治性出血的骨缺损。MQ T微粒表现出高效的可降解性、低细胞毒性和良好的血液相容性。在具有不同Q/T双层的各种MQ T微粒中，最外层为多酚层的MQT具有血小板粘附/活化和红细胞聚集的独特特性，从而产生最佳的止血性能。在小鼠松质骨缺损模型中，MQT表现出良好的止血效果、低炎症/免疫反应、高生物降解性和促进骨修复。对比格犬的概念验证研究进一步证实了MQT在控制骨缺损难治性出血方面的良好性能。目前的工作表明，这种基于生物质的多层结构微粒是非常有前途的临床用生物医学材料。
