Wang Yu, Li Xin, Chen Hanlu, Shi Yanfen, Li Yang, Zhang Guochao, Hu Yang, Xu Fu-Jian
State Key Laboratory of Chemical Resource Engineering, Key Lab of Biomedical Materials of Natural Macromolecules (Beijing University of Chemical Technology, Ministry of Education), Beijing Laboratory of Biomedical Materials, College of Materials Science and Engineering, Beijing University of Chemical Technology, Beijing, 100029, PR China.
Quzhou Institute for Innovation in Resource Chemical Engineering, Quzhou, 324000, PR China.
Mater Today Bio. 2025 Aug 5;34:102171. doi: 10.1016/j.mtbio.2025.102171. eCollection 2025 Oct.
Commercially available gelatin sponges are widely used in coagulation-dependent bleeding wounds due to its porous structure that concentrates blood cells, and coagulation factors. However, effective hemostasis cannot currently be achieved under coagulopathic conditions. In this study, we designed a procoagulant zein-polyphenol conjugate (ZC) nanoassemblies prepared using an anti-solvent strategy, were directly applied to the surface functionalization of commercially available gelatin sponges. Polyphenols give ZC coatings excellent adhesion and enhance the gelatin sponge's procoagulant properties. Leveraging their distinctive secondary structure, the optimized ZC nanoparticle-coated sponges demonstrated enhanced hemostatic properties compared to unmodified commercial gelatin sponges and exhibited superior red blood cell and platelet adhesion characteristics. Additionally, the sponges enhanced with the ZC coating exhibited superior hemostatic potential in a femoral-artery-injury model, under both coagulation-dependent and coagulopathic conditions. The current study presents a promising approach for the utilisation of zein-based procoagulant materials for versatile hemostatic materials engineering applications.
市售明胶海绵因其多孔结构可浓缩血细胞和凝血因子，而被广泛用于凝血依赖性出血伤口。然而，目前在凝血病条件下尚无法实现有效的止血。在本研究中，我们设计了一种采用反溶剂策略制备的促凝血玉米醇溶蛋白-多酚缀合物（ZC）纳米组装体，将其直接应用于市售明胶海绵的表面功能化。多酚赋予ZC涂层优异的附着力，并增强明胶海绵的促凝血性能。利用其独特的二级结构，优化后的ZC纳米颗粒涂层海绵与未改性的市售明胶海绵相比，具有增强的止血性能，并表现出优异的红细胞和血小板粘附特性。此外，在凝血依赖性和凝血病条件下，涂有ZC涂层的海绵在股动脉损伤模型中均表现出卓越的止血潜力。本研究为利用基于玉米醇溶蛋白的促凝血材料进行多功能止血材料工程应用提供了一种有前景的方法。
