Khine Nwai Oo, Lugsomya Kittitat, Kaewgun Benjarong, Honhanrob Lertrob, Pairojrit Panupong, Jermprasert Suthipat, Prapasarakul Nuvee
Department of Veterinary Microbiology, Faculty of Veterinary Sciences, Chulalongkorn University, Bangkok, Thailand.
The International Graduate Course of Veterinary Science and Technology, Chulalongkorn University, Bangkok, Thailand.
Front Vet Sci. 2020 Nov 10;7:582899. doi: 10.3389/fvets.2020.582899. eCollection 2020.
The presence of the plasmid-mediated colistin resistance encoding gene family in the is one of the crucial global concerns. The use of colistin in livestock rearing is believed to be the cause of gene spreading and is of impact to public health. The objective of this research was to detect the frequency and virulent genes of positive (MCRPE) in fecal samples from healthy pigs in a contract farming system across Thailand. A total of 696 pooled samples were derived from 80 farms, located in 49 provinces across six regions of Thailand. The colistin-resistant were identified by MALDI-TOF mass spectrometry and antimicrobial susceptibility testing by broth microdilution. The antibiogram was determined using an automated susceptibility machine, and the genetic characteristics were investigated for genes, phylogenetic group, replicon types, and virulent genes. In total, 31 of 696 samples were positive, with containing or combination of and with incidence of 4.45 and 0.43%. Phylogenetic groups A and B1 and the IncF and IncFIB replicon types were predominantly found in the MCRPE located in the central area, with multidrug-resistant traits against 3-14 types of antimicrobials. Additionally, 19 of 31 isolates identified as enterotoxigenic were with the and (enterotoxin-encoding genes). In conclusion, a low carriage rate of positive was detected in the large-scale farming of healthy pigs. The association between multidrug-resistant MCRPE and their pathogenic potential should be of concern.
质粒介导的黏菌素耐药编码基因家族的存在是全球关键关注问题之一。在牲畜饲养中使用黏菌素被认为是该基因传播的原因，并且对公共卫生有影响。本研究的目的是检测泰国合同制养殖系统中健康猪粪便样本中阳性多重耐药产超广谱β-内酰胺酶肠杆菌科细菌（MCRPE）的频率和毒力基因。总共696份混合样本来自泰国六个地区49个省份的80个农场。通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）和肉汤微量稀释法进行抗菌药物敏感性测试来鉴定耐黏菌素的肠杆菌科细菌。使用自动药敏仪确定抗菌谱，并研究其基因特征、系统发育群、复制子类型和毒力基因。总共696份样本中有31份呈阳性，其中含有mcr-1或mcr-1与mcr-2的组合，发生率分别为4.45%和0.43%。系统发育群A和B1以及IncF和IncFIB复制子类型主要存在于中部地区的MCRPE中，对3 - 14种抗菌药物具有多重耐药特性。此外，在31株被鉴定为产肠毒素的分离株中，有19株携带elt和est（肠毒素编码基因）。总之，在健康猪的大规模养殖中检测到阳性MCRPE的携带率较低。多重耐药MCRPE与其致病潜力之间的关联应受到关注。
