Dang Son Thi Thanh, Truong Duong Thi Quy, Olsen John Elmerdahl, Tran Nhat Thi, Truong Giang Thi Huong, Vu Hue Thi Kim, Dalsgaard Anders
National Institute of Veterinary Research, 74 Truong Chinh, Phuong Dinh, Dong Da, Hanoi, Vietnam.
Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Stigboejlen 4, Frederiksberg C 1870, Denmark.
FEMS Microbes. 2020 Oct 9;1(1):xtaa003. doi: 10.1093/femsmc/xtaa003. eCollection 2020 Sep.
WHO considers colistin as a highest priority critically important drug for human health, and occurrence of colistin-resistant bacteria in livestock is of health concern. The current study determined occurrence of colistin-resistant in pigs and workers at pig farms in Vietnam, and investigated the genetic background for resistance. Colistin-resistant were detected from pigs in 53/116 (45.7%) farms, and from workers taking care of the pigs in 21/94 (22.3%) farms. Colistin-resistant isolates showed MIC to colistin between 4-16 mg/L, they were multidrug resistant (99%) and resistance was caused by the presence of genes in 97/102 (95.1%) from pigs and in 31/34 (91.1%) isolates from humans. is considered a plasmid-encoded gene, but this was not confirmed in the current investigation. In total, one pig isolate carried both and genes, whereas and genes were not detected. Shared resistance profiles between pig and human isolates on the same farm was only observed in four farms. The study showed that commensal from pigs in Vietnam constitute a reservoir for colistin-resitant , however, further studies are needed to confirm that genes are associated with plasmids and their importance for human health.
世界卫生组织将黏菌素视为对人类健康极为重要的最高优先级药物，家畜中出现耐黏菌素细菌令人担忧。本研究确定了越南养猪场猪和工人中耐黏菌素的发生情况，并调查了耐药的遗传背景。在53/116（45.7%）个猪场的猪中检测到耐黏菌素，在21/94（22.3%）个猪场照顾猪的工人中检测到耐黏菌素。耐黏菌素分离株对黏菌素的最低抑菌浓度在4-16mg/L之间，它们对多种药物耐药（99%），耐药是由97/102（95.1%）来自猪的分离株和31/34（91.1%）来自人的分离株中存在mcr基因引起的。mcr被认为是一种质粒编码基因，但在本研究中未得到证实。总共，一株猪分离株同时携带mcr和mcr基因，而未检测到mcr和mcr基因。仅在四个猪场观察到同一猪场猪和人分离株之间的共同耐药谱。该研究表明，越南猪的共生菌构成了耐黏菌素mcr的储存库，然而，需要进一步研究以确认mcr基因与质粒的关联及其对人类健康的重要性。
