Yuan Shao-Ping, Li Chang-Xing, Qin Si, Wen Ju, Zhang Xi-Bao, Tian Xin, Zhu Chao-Ying, Li Ting, Huang Jin-Ping, Zheng Xiao-Huan
The Second School of Clinical Medicine, Southern Medical University, Guangzhou, China.
Department of Dermatology, Guangdong Second Provincial General Hospital, Guangzhou, China.
Ann Transl Med. 2020 Sep;8(18):1131. doi: 10.21037/atm-20-5067.
Disabled homolog 2-interacting protein (DAB2IP), a Ras GTPase-activating protein, is downregulated in several cancers. Its depletion is involved in tumor cell proliferation, apoptosis, and metastasis, as well as epithelial-mesenchymal transition. The present study aimed to explore the potential role of DAB2IP in cutaneous squamous cell carcinoma (cSCC) and provide a theoretical basis for the diagnosis and targeted therapy of cSCC.
The clinicopathological features of DAB2IP expression in cSCC were analyzed by immunohistochemistry, and the effects of DAB2IP on SCL-1 cell behavior were determined via genetic interference . SCL-1 cell lines that exhibited reduced expression of DAB2IP and a scrambled shRNA control were constructed using a lentivirus vector-based shRNA technique. RNA extraction, reverse transcription-quantitative PCR (RT-qPCR), MTT assay, colony formation test, cell cycle analysis, apoptosis test, transwell assay, wound-healing assay, invasive assay were used in this study.
The immunohistochemical results demonstrated that the expression of DAB2IP was higher in cSCC tissues than in soft fibroma. The level of DAB2IP expression was associated with the degree of malignancy and the depth of tumor infiltration; however, it had no association with patients' sex, tumor size, location, or phenotype. The results of the MTT, cell cycle, apoptosis, and invasion experiments demonstrated that knockdown of DAB2IP inhibited the viability and invasion of SCL-1 cells .
High expression of DAB2IP may contribute to the development and proliferation of cSCC.
Disabled同源2相互作用蛋白（DAB2IP）是一种Ras GTP酶激活蛋白，在多种癌症中表达下调。其缺失与肿瘤细胞增殖、凋亡、转移以及上皮-间质转化有关。本研究旨在探讨DAB2IP在皮肤鳞状细胞癌（cSCC）中的潜在作用，为cSCC的诊断和靶向治疗提供理论依据。
采用免疫组织化学分析cSCC中DAB2IP表达的临床病理特征，并通过基因干扰确定DAB2IP对SCL-1细胞行为的影响。使用基于慢病毒载体的shRNA技术构建DAB2IP表达降低的SCL-1细胞系和乱序shRNA对照。本研究采用RNA提取、逆转录定量PCR（RT-qPCR）、MTT法、集落形成试验、细胞周期分析、凋亡试验、Transwell试验、伤口愈合试验、侵袭试验。
免疫组织化学结果显示，cSCC组织中DAB--IP的表达高于软纤维瘤。DAB2IP的表达水平与恶性程度和肿瘤浸润深度相关；然而，它与患者的性别、肿瘤大小、位置或表型无关。MTT、细胞周期、凋亡和侵袭实验结果表明，敲低DAB2IP可抑制SCL-1细胞的活力和侵袭能力。
DAB2IP的高表达可能促进cSCC的发生和增殖。
