电纺聚氨酯纳米纤维和 3D 打印聚己内酯支架在大鼠模型中食管重建的实验研究。
Experimental investigation of esophageal reconstruction with electrospun polyurethane nanofiber and 3D printing polycaprolactone scaffolds using a rat model.
机构信息
Department of Otorhinolaryngology-Head & Neck Surgery, Samsung Changwon Hospital, Sungkyunkwan University School of Medicine, Changwon, South Korea.
Department of Otorhinolaryngology-Head and Neck Surgery, Seoul National University Hospital, Seoul, South Korea.
出版信息
Head Neck. 2021 Mar;43(3):833-848. doi: 10.1002/hed.26540. Epub 2020 Nov 26.
BACKGROUND
We evaluated the outcome of esophageal reconstructions using tissue-engineered scaffolds.
METHOD
Partial esophageal defects were reconstructed with the following scaffolds; animals were grouped (n = 7 per group) as follows: (a) normal rats; (b) rats implanted with three-dimensional printing (3DP) polycaprolactone (PCL) scaffolds; (c) with human adipose-derived mesenchymal stem cell (ADSC)-seeded 3DP PCL scaffolds; (d) with polyurethane (PU)-nanofiber(Nf) scaffolds; and (e) with ADSC-seeded PU-Nf scaffolds.
RESULTS
The esophageal defects were successfully repaired; however, muscle regeneration was greater in the 3DP PCL + ADSC groups than in the PU-Nf + ADSC groups (P < .001). Regeneration of the epithelium was greater in PU-Nf and PU-Nf + ADSC groups than in the 3DP PCL and 3DP PCL + ADSC groups (P < .001).
CONCLUSION
A tendency for more re-epithelization was observed with the PU-Nf scaffolds, while more muscle regeneration was achieved with the 3DP PCL scaffolds.
背景
我们评估了使用组织工程支架进行食管重建的结果。
方法
使用以下支架重建部分食管缺损；将动物分为以下几组（每组 7 只）：（a）正常大鼠；（b）植入三维打印（3DP）聚己内酯（PCL）支架的大鼠；（c）种植人脂肪间充质干细胞（ADSC）的 3DP PCL 支架；（d）聚氨酯（PU）纳米纤维（Nf）支架；和（e）种植 ADSC 的 PU-Nf 支架。
结果
食管缺损成功修复；然而，3DP PCL+ADSC 组的肌肉再生大于 PU-Nf+ADSC 组（P<.001）。上皮再生在 PU-Nf 和 PU-Nf+ADSC 组大于 3DP PCL 和 3DP PCL+ADSC 组（P<.001）。
结论
观察到 PU-Nf 支架具有更多的再上皮化倾向，而 3DP PCL 支架则实现了更多的肌肉再生。
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