沉默长链非编码RNA HIF1A-AS2抑制宫颈癌细胞的增殖、侵袭和迁移

[Silencing long non-coding RNA HIF1A-AS2 inhibits proliferation, invasion and migration of cervical cancer cells ].

作者信息

Deng Rong, Zhang Fuyun, Lei Fuzhen, Liu Wenjun, Liu Sisun, Wang Wenhua

机构信息

Department of Gynecology, General Hospital of Pingxiang Mining Group CO., Ltd., Pingxiang 337003, China, China.

Department of Gynecology, Pingxiang People's Hospital, Pingxiang 337000, China.

出版信息

Nan Fang Yi Ke Da Xue Xue Bao. 2020 Nov 30;40(11):1615-1621. doi: 10.12122/j.issn.1673-4254.2020.11.12.

DOI:10.12122/j.issn.1673-4254.2020.11.12

PMID:33243752

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7704379/

Abstract

OBJECTIVE

To explore the inhibitory effects of silencing long non-coding RNA (LncRNA) HIF1A-AS2 on epithelialmesenchymal transition (EMT) and tumor stem cell-like phenotype in cervical cancer cells.

METHODS

We designed 3 shRNA constructs for silencing HIF1A-AS2 in CaSki cells, and the shRNA with the strongest interference effect was selected for subsequent experiment. CaSki cells were transfected with shRNA-NC or Sh-HIF1A-AS2, and the changes in cell viability, invasion ability, EMT, expressions of EMT-related proteins, formation of cell spheres and expressions of stem cell markers were detected.

RESULTS

Transfection with shRNA-NC and Sh-HIF1A-AS2 did not significantly affected the viability of CaSki cells ( > 0.05). Compared with the cells transfected with shRNA-NC, the cells transfected with Sh- HIF1A-AS2 showed significantly reduced invasion ability, expressions of vimentin N-cadherin, and cell sphere formation ability. HIF1A-AS2 silencing obviously lowered the rate of ABCG2-positive cells, significantly reduced the mRNA and protein expressions of Nanog, OCT4, and SOX2, and strongly enhanced the expression of E-cadherin in CaSki cells ( < 0.05).

CONCLUSIONS

Silencing HIF1A-AS2 can inhibit proliferation, invasion and migration of cervical cancer cells .

摘要

目的

探讨沉默长链非编码RNA（LncRNA）HIF1A-AS2对宫颈癌细胞上皮间质转化（EMT）和肿瘤干细胞样表型的抑制作用。

方法

我们设计了3种用于沉默CaSki细胞中HIF1A-AS2的短发夹RNA（shRNA）构建体，并选择干扰效果最强的shRNA进行后续实验。用shRNA-NC或Sh-HIF1A-AS2转染CaSki细胞，检测细胞活力、侵袭能力、EMT、EMT相关蛋白表达、细胞球形成及干细胞标志物表达的变化。

结果

shRNA-NC和Sh-HIF1A-AS2转染对CaSki细胞活力无显著影响（>0.05）。与转染shRNA-NC的细胞相比，转染Sh-HIF1A-AS2的细胞侵袭能力、波形蛋白和N-钙黏蛋白表达及细胞球形成能力显著降低。HIF1A-AS2沉默明显降低了ABCG2阳性细胞率，显著降低了CaSki细胞中Nanog、OCT4和SOX2的mRNA和蛋白表达，并强烈增强了E-钙黏蛋白的表达（<0.05）。

结论

沉默HIF1A-AS2可抑制宫颈癌细胞的增殖、侵袭和迁移。

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